Axoplasmic transport of choline acetyltransferase activity in mice

effect of age and neurotomy

Research output: Contribution to journalArticle

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Abstract

The authors studied the axoplasmic transport of choline acetyltransferase (CAT) activity in sciatic nerves of normal mice of various ages. For at least 3 days after unilateral ligation of sciatic nerves of 6 and 30 wk old mice, the CAT activity in the ligated nerve increased as a linear function of time and the increase was confined to the 3 mm length of nerve immediately proximal to the ligature. The rate of increase of CAT activity in the ligated nerves of the 30 wk old mice was only 45 ± 6% that of the 6 wk old mice, whereas the CAT activity of non ligated sciatic nerves of the older mice was 87 ± 6% more than that of the younger mice (n = 18, P < 0.001). The average velocity of axoplasmic transport of CAT activity was 5 times greater in the younger mice (1.5 ± 0.2 mm/day vs 0.3 ± 0.1 mm/day, n = 6, P < 0.01). Even greater differences were observed between still younger and older animals: the av velocity of axoplasmic transport of 2 wk old mice (3.5 ± 0.2 mm/day) was 17.5 times greater than that of 36 wk old mice (0.2 ± 0.1 mm/day). They also studied the axoplasmic transport of CAT activity in 6 wk old mice after unilateral section of the sciatic nerve. For at least 3 mth after the operation, there were no differences between the sectioned nerves and the intact contralateral nerves with respect to the increase in CAT activity immediately proximal to a ligature placed at various times after neurotomy and one day before sacrifice. On the other hand, there was a reduction in the CAT activity of more proximal segments of the sectioned nerves. The reduction of CAT activity was maximal (52 ± 3%) 3 wk after the operation when the maximum increase (2.5 fold) in the av velocity of axoplasmic transport of CAT activity was recorded (n = 6, P < 0.001). The inclusion of purified (100 fold) mouse brain CAT activity in the assays for the AT activity of nerve segments demonstrated that the differences in content and rate of transport were not due to the presence of activators or inhibitors of CAT activity. These differences probably reflect physiologic changes in the axoplasmic transport of cholinergic neurons during development and regeneration.

Original languageEnglish (US)
Pages (from-to)583-593
Number of pages11
JournalJournal of Neurochemistry
Volume25
Issue number5
StatePublished - 1975

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Axonal Transport
Choline O-Acetyltransferase
Sciatic Nerve
Ligation
Cholinergic Neurons
Cholinergic Agents
Neurons
Regeneration
Assays
Brain
Animals

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Axoplasmic transport of choline acetyltransferase activity in mice : effect of age and neurotomy. / Jablecki, C.; Brimijoin, William Stephen.

In: Journal of Neurochemistry, Vol. 25, No. 5, 1975, p. 583-593.

Research output: Contribution to journalArticle

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title = "Axoplasmic transport of choline acetyltransferase activity in mice: effect of age and neurotomy",
abstract = "The authors studied the axoplasmic transport of choline acetyltransferase (CAT) activity in sciatic nerves of normal mice of various ages. For at least 3 days after unilateral ligation of sciatic nerves of 6 and 30 wk old mice, the CAT activity in the ligated nerve increased as a linear function of time and the increase was confined to the 3 mm length of nerve immediately proximal to the ligature. The rate of increase of CAT activity in the ligated nerves of the 30 wk old mice was only 45 ± 6{\%} that of the 6 wk old mice, whereas the CAT activity of non ligated sciatic nerves of the older mice was 87 ± 6{\%} more than that of the younger mice (n = 18, P < 0.001). The average velocity of axoplasmic transport of CAT activity was 5 times greater in the younger mice (1.5 ± 0.2 mm/day vs 0.3 ± 0.1 mm/day, n = 6, P < 0.01). Even greater differences were observed between still younger and older animals: the av velocity of axoplasmic transport of 2 wk old mice (3.5 ± 0.2 mm/day) was 17.5 times greater than that of 36 wk old mice (0.2 ± 0.1 mm/day). They also studied the axoplasmic transport of CAT activity in 6 wk old mice after unilateral section of the sciatic nerve. For at least 3 mth after the operation, there were no differences between the sectioned nerves and the intact contralateral nerves with respect to the increase in CAT activity immediately proximal to a ligature placed at various times after neurotomy and one day before sacrifice. On the other hand, there was a reduction in the CAT activity of more proximal segments of the sectioned nerves. The reduction of CAT activity was maximal (52 ± 3{\%}) 3 wk after the operation when the maximum increase (2.5 fold) in the av velocity of axoplasmic transport of CAT activity was recorded (n = 6, P < 0.001). The inclusion of purified (100 fold) mouse brain CAT activity in the assays for the AT activity of nerve segments demonstrated that the differences in content and rate of transport were not due to the presence of activators or inhibitors of CAT activity. These differences probably reflect physiologic changes in the axoplasmic transport of cholinergic neurons during development and regeneration.",
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N2 - The authors studied the axoplasmic transport of choline acetyltransferase (CAT) activity in sciatic nerves of normal mice of various ages. For at least 3 days after unilateral ligation of sciatic nerves of 6 and 30 wk old mice, the CAT activity in the ligated nerve increased as a linear function of time and the increase was confined to the 3 mm length of nerve immediately proximal to the ligature. The rate of increase of CAT activity in the ligated nerves of the 30 wk old mice was only 45 ± 6% that of the 6 wk old mice, whereas the CAT activity of non ligated sciatic nerves of the older mice was 87 ± 6% more than that of the younger mice (n = 18, P < 0.001). The average velocity of axoplasmic transport of CAT activity was 5 times greater in the younger mice (1.5 ± 0.2 mm/day vs 0.3 ± 0.1 mm/day, n = 6, P < 0.01). Even greater differences were observed between still younger and older animals: the av velocity of axoplasmic transport of 2 wk old mice (3.5 ± 0.2 mm/day) was 17.5 times greater than that of 36 wk old mice (0.2 ± 0.1 mm/day). They also studied the axoplasmic transport of CAT activity in 6 wk old mice after unilateral section of the sciatic nerve. For at least 3 mth after the operation, there were no differences between the sectioned nerves and the intact contralateral nerves with respect to the increase in CAT activity immediately proximal to a ligature placed at various times after neurotomy and one day before sacrifice. On the other hand, there was a reduction in the CAT activity of more proximal segments of the sectioned nerves. The reduction of CAT activity was maximal (52 ± 3%) 3 wk after the operation when the maximum increase (2.5 fold) in the av velocity of axoplasmic transport of CAT activity was recorded (n = 6, P < 0.001). The inclusion of purified (100 fold) mouse brain CAT activity in the assays for the AT activity of nerve segments demonstrated that the differences in content and rate of transport were not due to the presence of activators or inhibitors of CAT activity. These differences probably reflect physiologic changes in the axoplasmic transport of cholinergic neurons during development and regeneration.

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