Axonal regulation of Schwann cell glycolipid biosynthesis

J. K. Yao, Anthony John Windebank, J. F. Poduslo, J. E. Yoshino

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (GalCe), monogalactosyl diacylglycerol (MGDG), and the sulfatides (Su). In contrast, the incorporation of [3H]galactose into MGDG, GalCe, and Su was nearly undetected in endoneurial explants after 4 days in vitro (div). Instead, there was increased 3H-labeling of glucocerebrosides (GlcCe) and its homologues, with tetrahexosylceramides (GL-4) being a major product, which continued through 8 div. This shift in glycolipid biosynthesis was further demonstrated in the purified Schwann cell cultures. These observations, together with our early findings in the permanent transection paradigm support a direct role of axons in specifying Schwann cell biosynthesis of the GalCe, MGDG, and Su and that the absence of this Schwann cell-axon interaction results in the phenotypic expression of glucocerebroside homologues by the Schwann cell.

Original languageEnglish (US)
Pages (from-to)279-282
Number of pages4
JournalNeurochemical Research
Volume15
Issue number3
StatePublished - 1990

Fingerprint

Schwann Cells
Glycolipids
Biosynthesis
Galactosylceramides
Sulfoglycosphingolipids
Cell culture
Cells
Diglycerides
Galactose
Glucosylceramides
Axons
Labeling
Sciatic Nerve
Rats
Cell Communication
Cell Culture Techniques
In Vitro Techniques

Keywords

  • [H]galactose
  • galactocerebroside
  • Glucocerebroside
  • nerve explants
  • peripheral nerve
  • Schwann cells

ASJC Scopus subject areas

  • Biochemistry
  • Neuroscience(all)

Cite this

Yao, J. K., Windebank, A. J., Poduslo, J. F., & Yoshino, J. E. (1990). Axonal regulation of Schwann cell glycolipid biosynthesis. Neurochemical Research, 15(3), 279-282.

Axonal regulation of Schwann cell glycolipid biosynthesis. / Yao, J. K.; Windebank, Anthony John; Poduslo, J. F.; Yoshino, J. E.

In: Neurochemical Research, Vol. 15, No. 3, 1990, p. 279-282.

Research output: Contribution to journalArticle

Yao, JK, Windebank, AJ, Poduslo, JF & Yoshino, JE 1990, 'Axonal regulation of Schwann cell glycolipid biosynthesis', Neurochemical Research, vol. 15, no. 3, pp. 279-282.
Yao, J. K. ; Windebank, Anthony John ; Poduslo, J. F. ; Yoshino, J. E. / Axonal regulation of Schwann cell glycolipid biosynthesis. In: Neurochemical Research. 1990 ; Vol. 15, No. 3. pp. 279-282.
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