Avian sarcoma and leukosis virus envelope glycoproteins evolve to broaden receptor usage under pressure from entry competitors

Audelia Munguia, Mark J. Federspiel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The subgroup A through E avian sarcoma and leukosis viruses (ASLV(A) through ASLV(E)) are a group of highly related alpharetroviruses that have evolved their envelope glycoproteins to use different receptors to enable efficient virus entry due to host resistance and/or to expand host range. Previously, we demonstrated that ASLV(A) in the presence of a competitor to the subgroup A Tva receptor, SUA-rIgG immunoadhesin, evolved to use other receptor options. The selected mutant virus, RCASBP(A)∆155–160, modestly expanded its use of the Tvb and Tvc receptors and possibly other cell surface proteins while maintaining the binding affinity to Tva. In this study, we further evolved the ∆155–160 virus with the genetic selection pressure of a soluble form of the Tva receptor that should force the loss of Tva binding affinity in the presence of the ∆155–160 mutation. Viable ASLVs were selected that acquired additional mutations in the ∆155–160 Env hypervariable regions that significantly broadened receptor usage to include Tvb and Tvc as well as retaining the use of Tva as a receptor determined by receptor interference assays. A similar deletion in the hr1 hypervariable region of the subgroup C ASLV glycoproteins evolved to broaden receptor usage when selected on Tvc-negative cells.

Original languageEnglish (US)
Article number519
JournalViruses
Volume11
Issue number6
DOIs
StatePublished - Jun 2019

Keywords

  • Genetic selection inhibiting entry
  • Receptor use expansion
  • The subgroup A through E avian sarcoma and leukosis viruses

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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