Automation of hybridization and capture based next generation sequencing library preparation requires reduction of on-deck bead binding and heated wash temperatures

Eric Zimmerman Zuckerman, Joseph A. Thompson, Amber R. Schneider, Michael B. Campion, Jennifer J. Johns, Theodore J. Stier, Lisa M. Peterson, Amanda M. Ward, Joseph H. Blommel, Rohan D. Gnanaolivu, Kimberly P. Lauer, Gopinath Sivasankaran, Jagadheshwar Balan, Surendra Dasari, Yuta Sakai, Cherisse A. Marcou, Gang Zheng, Kevin Carl Halling, Wei Shen, David S. ViswanathaZhiyv Niu

Research output: Contribution to journalArticlepeer-review

Abstract

Capture-based library preparation for next generation sequencing (NGS) offers a balance between sequencing depth and bioinformatics cost of analysis. Liquid handling automation enhances the reliability of the library preparation process by reducing sample-to-sample variation and substantially enhances throughput, particularly when it can be employed in a 'walk-away' fashion with limited hands-on interaction. This requires complex series of mixing and heating steps like those utilized in capture chemistries to happen on the liquid handler. While developing liquid handling automation for Integrated DNA Technologies (IDT) xGen Exome, Illumina TruSight Oncology 500, and Personal Genome Diagnostics (PGDx) elio Plasma Resolve chemistries on the PerkinElmer Sciclone liquid handler, we found that applying the capture temperatures recommended for manual library preparation results in low yield on automation. To restore the final library yield, we reduced bead binding and/or heated wash temperatures of the Peltier heaters on the liquid handlers by about 10°C. Since this applied across three unique capture-based chemistries, we consider this a generalizable principle of automating capture on the Sciclone. We hypothesize that this is driven by the very different thermodynamic environments represented by a sealed plate on a thermal cycler and a plate with a lid on a Peltier heater. This phenomenon should be considered when automating NGS library preparation on PerkinElmer Sciclone instruments.

Original languageEnglish (US)
Pages (from-to)214-218
Number of pages5
JournalSLAS Technology
Volume27
Issue number3
DOIs
StatePublished - Jun 1 2022

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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