@inproceedings{41ec92286bac471689a8c030091e3662,
title = "Automated sample preparation for nucleosome positioning analysis using droplet microfluidics",
abstract = "Automated nucleosome preparation is accomplished for nucleosome positioning analysis on a droplet microfluidic device. The designed platform encapsulates cells and enzymes into droplets continuously, allowing flexible sizes of cell samples to be processed into nucleosomal DNA with no user intervention. The quality of digested DNA from the device is validated by next-generation sequencing to show distinct nucleosome distribution patterns at different gene regions. The droplet microfluidic device presented here demonstrates an effective way to extend the application of nucleosome positioning assay to clinical samples and the potential to facilitate chromatin-based precision diagnosis and treatment.",
keywords = "Droplet Microfluidics, Nucleosome Positioning, Precision Diagnosis, Treatment",
author = "Yi Xu and Lee, {Jeong Heon} and Yongbing Zhao and Zhaoyu Li and Tamas Ordog and Bailey, {Ryan C.}",
note = "Publisher Copyright: {\textcopyright} 17CBMS-0001.; 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",
year = "2020",
language = "English (US)",
series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
publisher = "Chemical and Biological Microsystems Society",
pages = "213--214",
booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
}