Automated sample preparation for nucleosome positioning analysis using droplet microfluidics

Yi Xu, Jeong Heon Lee, Yongbing Zhao, Zhaoyu Li, Tamas Ordog, Ryan C. Bailey

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Automated nucleosome preparation is accomplished for nucleosome positioning analysis on a droplet microfluidic device. The designed platform encapsulates cells and enzymes into droplets continuously, allowing flexible sizes of cell samples to be processed into nucleosomal DNA with no user intervention. The quality of digested DNA from the device is validated by next-generation sequencing to show distinct nucleosome distribution patterns at different gene regions. The droplet microfluidic device presented here demonstrates an effective way to extend the application of nucleosome positioning assay to clinical samples and the potential to facilitate chromatin-based precision diagnosis and treatment.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages213-214
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Droplet Microfluidics
  • Nucleosome Positioning
  • Precision Diagnosis
  • Treatment

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Xu, Y., Lee, J. H., Zhao, Y., Li, Z., Ordog, T., & Bailey, R. C. (2020). Automated sample preparation for nucleosome positioning analysis using droplet microfluidics. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 213-214). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.