Automated assessment of keratocyte density in clinical confocal microscopy of the corneal stroma

J. W. McLaren, S. V. Patel, C. B. Nau, W. M. Bourne

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cell density in the corneal stroma is typically determined by counting the number of bright objects, presumably keratocyte nuclei, in images from clinical confocal microscopy. We present a program that identifies bright objects and counts those that most likely represent cells. Selection variables were determined from 125 normal corneas with cell densities that had been assessed manually. The program was tested on 17 corneas of patients before and at several intervals to 5 years after laser in situ keratomileusis (LASIK) surgery. In these corneas, which showed a decrease in cell density after surgery, the program identified cells as well as human observers did.

Original languageEnglish (US)
Pages (from-to)21-31
Number of pages11
JournalJournal of Microscopy
Volume229
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Corneal Stroma
Confocal microscopy
Confocal Microscopy
Cornea
Surgery
Cell Count
cornea
microscopy
cells
Laser In Situ Keratomileusis
surgery
Lasers
counting
intervals
nuclei
lasers

Keywords

  • Cell density
  • Confocal microscopy
  • Cornea
  • Image processing
  • Keratocyte
  • Keratocyte density

ASJC Scopus subject areas

  • Instrumentation

Cite this

Automated assessment of keratocyte density in clinical confocal microscopy of the corneal stroma. / McLaren, J. W.; Patel, S. V.; Nau, C. B.; Bourne, W. M.

In: Journal of Microscopy, Vol. 229, No. 1, 01.2008, p. 21-31.

Research output: Contribution to journalArticle

McLaren, J. W. ; Patel, S. V. ; Nau, C. B. ; Bourne, W. M. / Automated assessment of keratocyte density in clinical confocal microscopy of the corneal stroma. In: Journal of Microscopy. 2008 ; Vol. 229, No. 1. pp. 21-31.
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