Automated assessment of keratocyte density in clinical confocal microscopy of the corneal stroma

J. W. McLaren, S. V. Patel, C. B. Nau, W. M. Bourne

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Cell density in the corneal stroma is typically determined by counting the number of bright objects, presumably keratocyte nuclei, in images from clinical confocal microscopy. We present a program that identifies bright objects and counts those that most likely represent cells. Selection variables were determined from 125 normal corneas with cell densities that had been assessed manually. The program was tested on 17 corneas of patients before and at several intervals to 5 years after laser in situ keratomileusis (LASIK) surgery. In these corneas, which showed a decrease in cell density after surgery, the program identified cells as well as human observers did.

Original languageEnglish (US)
Pages (from-to)21-31
Number of pages11
JournalJournal of Microscopy
Volume229
Issue number1
DOIs
StatePublished - Jan 1 2008

Keywords

  • Cell density
  • Confocal microscopy
  • Cornea
  • Image processing
  • Keratocyte
  • Keratocyte density

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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