Autoimmunoreactive IgGs against cardiac lipid raft-associated proteins in patients with postural orthostatic tachycardia syndrome

Xiao Li Wang, Tian You Ling, M. Cristine Charlesworth, Juan J. Figueroa, Phillip Anson Low, Win Kuang Shen, Hon Chi Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Lipid rafts are specialized plasma membrane microdomains that serve as platforms for integrating cellular signal transductions. We have recently reported that autoantibodies against cardiac membrane proteins are present in patients with postural orthostatic tachycardia syndrome (POTS). In this study, we examined the presence of autoimmunoreactive IgGs against lipid raft proteins in these patients. IgGs were purified from the sera of 10 patients and 7 normal controls. Cardiac lipid raft preparations were isolated from normal human heart tissue. The lipid raft-associated proteins were resolved by 2-dimensional gel electrophoresis and immunoblotted against IgGs from each subject. Protein spots that reacted specifically with patient IgGs were identified by nano-liquid chromatography-mass spectrometry/mass spectrometry. Thirty-four such protein spots, and 72 unique proteins were identified. The targets of autoimmunoreactive IgGs include proteins associated with caveolae structure, adrenergic signaling, calcium signaling, cytostructures, chaperone and energy metabolism. Multiple pathways were involved including those that regulate caveolae-mediated signaling, oxidative phosphorylation, fatty acid metabolism, protein ubiquitination, and cardiac β-adrenergic signaling. Our results suggest that cardiac lipid raft-associated proteins are targets of autoimmunoreactive IgGs from patients with POTS. Autoimmunity may play a role in the pathogenesis of POTS.

Original languageEnglish (US)
Pages (from-to)34-44
Number of pages11
JournalTranslational Research
Volume162
Issue number1
DOIs
StatePublished - Jul 2013

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Postural Orthostatic Tachycardia Syndrome
Lipids
Proteins
Caveolae
Adrenergic Agents
Mass spectrometry
Membrane Microdomains
Signal transduction
Calcium Signaling
Ubiquitination
Oxidative Phosphorylation
Liquid chromatography
Cell membranes
Tandem Mass Spectrometry
Electrophoresis
Autoimmunity
Metabolism
Liquid Chromatography
Autoantibodies
Energy Metabolism

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, medical
  • Public Health, Environmental and Occupational Health

Cite this

Autoimmunoreactive IgGs against cardiac lipid raft-associated proteins in patients with postural orthostatic tachycardia syndrome. / Wang, Xiao Li; Ling, Tian You; Charlesworth, M. Cristine; Figueroa, Juan J.; Low, Phillip Anson; Shen, Win Kuang; Lee, Hon Chi.

In: Translational Research, Vol. 162, No. 1, 07.2013, p. 34-44.

Research output: Contribution to journalArticle

Wang, Xiao Li ; Ling, Tian You ; Charlesworth, M. Cristine ; Figueroa, Juan J. ; Low, Phillip Anson ; Shen, Win Kuang ; Lee, Hon Chi. / Autoimmunoreactive IgGs against cardiac lipid raft-associated proteins in patients with postural orthostatic tachycardia syndrome. In: Translational Research. 2013 ; Vol. 162, No. 1. pp. 34-44.
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