Autocrine Tnf signaling favors malignant cells in myelofibrosis in a Tnfr2-dependent fashion

William L. Heaton, Anna V. Senina, Anthony D. Pomicter, Mohamed Salama, Phillip M. Clair, Dongqing Yan, Russell N. Bell, Jeremy M. Gililland, Josef T. Prchal, Thomas O’Hare, Michael W. Deininger

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tumor necrosis factor alpha (TNF) is increased in myelofibrosis (MF) and promotes survival of malignant over normal cells. The mechanisms altering TNF responsiveness in MF cells are unknown. We show that the proportion of marrow (BM) cells expressing TNF is increased in MF compared to controls, with the largest differential in primitive cells. Blockade of TNF receptor 2 (TNFR2), but not TNFR1, selectively inhibited colony formation by MF CD34 + and mouse JAK2 V617F progenitor cells. Microarray of mouse MPN revealed reduced expression of X-linked inhibitor of apoptosis (Xiap) and mitogen-activated protein kinase 8 (Mapk8) in JAK2 V617F relative to JAK2 WT cells, which were normalized by TNFR2 but not TNFR1 blockade. XIAP and MAPK8 were also reduced in MF CD34 + cells compared to normal BM, and their ectopic expression induced apoptosis. Unlike XIAP, expression of cellular IAP (cIAP) protein was increased in MF CD34 + cells. Consistent with cIAP’s role in NF-κB activation, TNF-induced NF-κB activity was higher in MF vs. normal BM CD34 + cells. This suggests that JAK2 V617F reprograms TNF response toward survival by downregulating XIAP and MAPK8 through TNFR2. Our results reveal an unexpected pro-apoptotic role for XIAP in MF and identify TNFR2 as a key mediator of TNF-induced clonal expansion.

Original languageEnglish (US)
Pages (from-to)2399-2411
Number of pages13
JournalLeukemia
Volume32
Issue number11
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

Fingerprint

Autocrine Communication
Primary Myelofibrosis
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor Receptors
Receptors, Tumor Necrosis Factor, Type I
Mitogen-Activated Protein Kinase 8
Receptors, Tumor Necrosis Factor, Type II
Apoptosis
Stem Cells
Down-Regulation
Bone Marrow

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Heaton, W. L., Senina, A. V., Pomicter, A. D., Salama, M., Clair, P. M., Yan, D., ... Deininger, M. W. (2018). Autocrine Tnf signaling favors malignant cells in myelofibrosis in a Tnfr2-dependent fashion. Leukemia, 32(11), 2399-2411. https://doi.org/10.1038/s41375-018-0131-z

Autocrine Tnf signaling favors malignant cells in myelofibrosis in a Tnfr2-dependent fashion. / Heaton, William L.; Senina, Anna V.; Pomicter, Anthony D.; Salama, Mohamed; Clair, Phillip M.; Yan, Dongqing; Bell, Russell N.; Gililland, Jeremy M.; Prchal, Josef T.; O’Hare, Thomas; Deininger, Michael W.

In: Leukemia, Vol. 32, No. 11, 01.11.2018, p. 2399-2411.

Research output: Contribution to journalArticle

Heaton, WL, Senina, AV, Pomicter, AD, Salama, M, Clair, PM, Yan, D, Bell, RN, Gililland, JM, Prchal, JT, O’Hare, T & Deininger, MW 2018, 'Autocrine Tnf signaling favors malignant cells in myelofibrosis in a Tnfr2-dependent fashion', Leukemia, vol. 32, no. 11, pp. 2399-2411. https://doi.org/10.1038/s41375-018-0131-z
Heaton, William L. ; Senina, Anna V. ; Pomicter, Anthony D. ; Salama, Mohamed ; Clair, Phillip M. ; Yan, Dongqing ; Bell, Russell N. ; Gililland, Jeremy M. ; Prchal, Josef T. ; O’Hare, Thomas ; Deininger, Michael W. / Autocrine Tnf signaling favors malignant cells in myelofibrosis in a Tnfr2-dependent fashion. In: Leukemia. 2018 ; Vol. 32, No. 11. pp. 2399-2411.
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abstract = "Tumor necrosis factor alpha (TNF) is increased in myelofibrosis (MF) and promotes survival of malignant over normal cells. The mechanisms altering TNF responsiveness in MF cells are unknown. We show that the proportion of marrow (BM) cells expressing TNF is increased in MF compared to controls, with the largest differential in primitive cells. Blockade of TNF receptor 2 (TNFR2), but not TNFR1, selectively inhibited colony formation by MF CD34 + and mouse JAK2 V617F progenitor cells. Microarray of mouse MPN revealed reduced expression of X-linked inhibitor of apoptosis (Xiap) and mitogen-activated protein kinase 8 (Mapk8) in JAK2 V617F relative to JAK2 WT cells, which were normalized by TNFR2 but not TNFR1 blockade. XIAP and MAPK8 were also reduced in MF CD34 + cells compared to normal BM, and their ectopic expression induced apoptosis. Unlike XIAP, expression of cellular IAP (cIAP) protein was increased in MF CD34 + cells. Consistent with cIAP’s role in NF-κB activation, TNF-induced NF-κB activity was higher in MF vs. normal BM CD34 + cells. This suggests that JAK2 V617F reprograms TNF response toward survival by downregulating XIAP and MAPK8 through TNFR2. Our results reveal an unexpected pro-apoptotic role for XIAP in MF and identify TNFR2 as a key mediator of TNF-induced clonal expansion.",
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AU - Salama, Mohamed

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AU - Yan, Dongqing

AU - Bell, Russell N.

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AU - Deininger, Michael W.

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