Autoantibodies to DEK oncoprotein in human inflammatory disease

Xingwen Dong, Jingsong Wang, Fathima N. Kabir, Melody Shaw, Ann M. Reed, Leonard Stein, Luis E.C. Andrade, Virginia F.M. Trevisani, Michael L. Miller, Takao Fujii, Masashi Akizuki, Lauren M. Pachman, Minoru Satoh, Westley H. Reeves

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA). Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation studies using baculovirus-expressed human DEK. Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21). Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. In general, levels of anti- DEK autoantibodies were higher in SLE than in other patient subsets, including JRA. Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-γ production rather than a particular disease subset.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalArthritis and rheumatism
Volume43
Issue number1
DOIs
StatePublished - Jan 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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