Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells

Seiga Ohmine, Jeffrey L Salisbury, James Ingle, Giuseppe Pettinato, Candace L. Haddox, Tufia C Haddad, Evanthia Galanis, Yasuhiro H Ikeda, Antonio D'Assoro

Research output: Contribution to journalArticle

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Abstract

The discovery of human induced pluripotent stem cells (hiPSCs) is a promising advancement in the field of regenerative and personalized medicine. Expression of SOX2, KLF4, OCT4 and MYC transcription factors induces the nuclear reprogramming of somatic cells into hiPSCs that share striking similarities with human embryonic stem cells (hESCs). However, several studies have demonstrated that hESCs and hiPSCs could lead to teratoma formation in vivo, thus limiting their current clinical applications. Aberrant cell cycle regulation of hESCs is linked to centrosome amplification, which may account, for their enhanced chromosomal instability (CIN), and thus increase their tumorigenicity. Significantly, the tumor suppressor p53 plays a key role as a 'guardian of reprogramming', safeguarding genomic integrity during hiPSC reprogramming. Nevertheless, the molecular mechanisms leading to development of CIN during reprogramming and increased tumorigenic potential of hiPSCs remains to be fully elucidated. In the present study, we analyzed CIN in hiPSCs derived from keratinocytes and established that chromosomal and mitotic aberrations were linked to centrosome amplification, Aurora-A overexpression, abrogation of p53-mediated G1/S cell cycle checkpoint and loss of Rb tumor-suppressor function. When hiPSCs were transplanted into the kidney capsules of immunocompromised mice, they developed high-grade teratomas characterized by the presence of cells that exhibited non-uniform shapes and sizes, high nuclear pleomorphism and centrosome amplification. Significantly, ex vivo cells derived from teratomas exhibited high self-renewal capacity that was linked to Aurora-A kinase activity and gave rise to lung metastasis when injected into the tail vein of immunocompromised mice. Collectively, these findings demonstrated a high risk for malignancy of hiPSCs that exhibit Aurora-A overexpression, loss of Rb function, centrosome amplification and CIN. Based on these findings, we proposed that Aurora-A-Targeted therapy could represent a promising prophylactic therapeutic strategy to decrease the likelihood of CIN and development of aggressive teratomas derived from hiPSCs.

Original languageEnglish (US)
Pages (from-to)1725-1730
Number of pages6
JournalOncology Reports
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2018

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Induced Pluripotent Stem Cells
Teratoma
Chromosomal Instability
Centrosome
Aurora Kinase A
G1 Phase Cell Cycle Checkpoints
Neoplasms
Precision Medicine
Regenerative Medicine
Keratinocytes
Chromosome Aberrations
Capsules
Tail
Veins
Cell Cycle
Transcription Factors
Neoplasm Metastasis
Kidney
Lung

Keywords

  • Aurora-A
  • Cell cycle checkpoint
  • HiPSCs
  • Metastasis
  • Teratoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells. / Ohmine, Seiga; Salisbury, Jeffrey L; Ingle, James; Pettinato, Giuseppe; Haddox, Candace L.; Haddad, Tufia C; Galanis, Evanthia; Ikeda, Yasuhiro H; D'Assoro, Antonio.

In: Oncology Reports, Vol. 39, No. 4, 01.04.2018, p. 1725-1730.

Research output: Contribution to journalArticle

Ohmine, Seiga ; Salisbury, Jeffrey L ; Ingle, James ; Pettinato, Giuseppe ; Haddox, Candace L. ; Haddad, Tufia C ; Galanis, Evanthia ; Ikeda, Yasuhiro H ; D'Assoro, Antonio. / Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells. In: Oncology Reports. 2018 ; Vol. 39, No. 4. pp. 1725-1730.
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AU - Ingle, James

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AU - Haddox, Candace L.

AU - Haddad, Tufia C

AU - Galanis, Evanthia

AU - Ikeda, Yasuhiro H

AU - D'Assoro, Antonio

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