Augmented growth hormone (GH) secretory burst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men

Mark L. Hartman, Johannes D Veldhuis, Michael L. Johnson, Mary M. Lee, K. G M M Alberti, Eugene Samojlik, Michael O. Thorner

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Abstract

Serum GH concentrations are increased in fasted or malnourished human subjects. We investigated the dynamic mechanisms underlying this phenomenon in nine normal men by analyzing serum GH concentrations measured in blood obtained at 5-min intervals over 24 h on a control (fed) day and on the second day of a fast with a multiple-parameter deconvolution method to simultaneously resolve endogenous GH secretory and clearance rates. Two days of fasting induced a 5-fold increase in the 24-h endogenous GH production rate [78 ± 12 vs. 371 ± 57 μg/Lv (Lv, liter of distribution volume) or 0.24 ± 0.038 vs. 1.1 ± 0.16 mg/m2 (assuming a distribution volume of 7.9% body weight), P = 0.0001]. This enhanced GH production rate was accounted for by 2-fold increases in the number of GH secretory bursts per 24 h (14 ± 2.3 us. 32 ± 2.4, P = 0.0006) and the mass of GH secreted per burst (6.3 ± 1.2 vs. 11 ± 1.6 μg/Lv, P = 0.002). The latter was a result of increased secretory-event amplitudes (maximal rates of GH release attained within a burst) with unchanged secretory burst durations. GH was secreted in complex volleys composed of multiple discrete secretory bursts. These secretory volleys were separated by shorter intervals of secretory quiescence in the fasted than fed state (respectively, 88 ± 4.2 us. 143 ± 14 min, P = 0.0001). Similarly, within volleys of GH release, constituent individual secretory bursts occurred more frequently during the fast [every 33 ± 0.64 (fasted) vs. every 44 ± 2.0 min (fed), P = 0.0001]. The t1/2 of endogenous GH was not significantly altered by fasting [18 ± 2.2 (fasted) vs. 20 ± 1.5 min (fed), P = 0.47]. Serum insulin-like growth factor I concentrations were unchanged after 56 h of fasting. In conclusion, the present data suggest that starvation-induced enhancement of GH secretion is mediated by an increased frequency of GHRH release, and longer and more pronounced periods of somatostatin withdrawal.

Original languageEnglish (US)
Pages (from-to)757-765
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume74
Issue number4
StatePublished - Apr 1992
Externally publishedYes

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Growth Hormone
Fasting
Serum
Secretory Rate
Deconvolution
Starvation
Somatostatin
Insulin-Like Growth Factor I
Blood
Body Weight

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Hartman, M. L., Veldhuis, J. D., Johnson, M. L., Lee, M. M., Alberti, K. G. M. M., Samojlik, E., & Thorner, M. O. (1992). Augmented growth hormone (GH) secretory burst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men. Journal of Clinical Endocrinology and Metabolism, 74(4), 757-765.

Augmented growth hormone (GH) secretory burst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men. / Hartman, Mark L.; Veldhuis, Johannes D; Johnson, Michael L.; Lee, Mary M.; Alberti, K. G M M; Samojlik, Eugene; Thorner, Michael O.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 74, No. 4, 04.1992, p. 757-765.

Research output: Contribution to journalArticle

Hartman, ML, Veldhuis, JD, Johnson, ML, Lee, MM, Alberti, KGMM, Samojlik, E & Thorner, MO 1992, 'Augmented growth hormone (GH) secretory burst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men', Journal of Clinical Endocrinology and Metabolism, vol. 74, no. 4, pp. 757-765.
Hartman, Mark L. ; Veldhuis, Johannes D ; Johnson, Michael L. ; Lee, Mary M. ; Alberti, K. G M M ; Samojlik, Eugene ; Thorner, Michael O. / Augmented growth hormone (GH) secretory burst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men. In: Journal of Clinical Endocrinology and Metabolism. 1992 ; Vol. 74, No. 4. pp. 757-765.
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abstract = "Serum GH concentrations are increased in fasted or malnourished human subjects. We investigated the dynamic mechanisms underlying this phenomenon in nine normal men by analyzing serum GH concentrations measured in blood obtained at 5-min intervals over 24 h on a control (fed) day and on the second day of a fast with a multiple-parameter deconvolution method to simultaneously resolve endogenous GH secretory and clearance rates. Two days of fasting induced a 5-fold increase in the 24-h endogenous GH production rate [78 ± 12 vs. 371 ± 57 μg/Lv (Lv, liter of distribution volume) or 0.24 ± 0.038 vs. 1.1 ± 0.16 mg/m2 (assuming a distribution volume of 7.9{\%} body weight), P = 0.0001]. This enhanced GH production rate was accounted for by 2-fold increases in the number of GH secretory bursts per 24 h (14 ± 2.3 us. 32 ± 2.4, P = 0.0006) and the mass of GH secreted per burst (6.3 ± 1.2 vs. 11 ± 1.6 μg/Lv, P = 0.002). The latter was a result of increased secretory-event amplitudes (maximal rates of GH release attained within a burst) with unchanged secretory burst durations. GH was secreted in complex volleys composed of multiple discrete secretory bursts. These secretory volleys were separated by shorter intervals of secretory quiescence in the fasted than fed state (respectively, 88 ± 4.2 us. 143 ± 14 min, P = 0.0001). Similarly, within volleys of GH release, constituent individual secretory bursts occurred more frequently during the fast [every 33 ± 0.64 (fasted) vs. every 44 ± 2.0 min (fed), P = 0.0001]. The t1/2 of endogenous GH was not significantly altered by fasting [18 ± 2.2 (fasted) vs. 20 ± 1.5 min (fed), P = 0.47]. Serum insulin-like growth factor I concentrations were unchanged after 56 h of fasting. In conclusion, the present data suggest that starvation-induced enhancement of GH secretion is mediated by an increased frequency of GHRH release, and longer and more pronounced periods of somatostatin withdrawal.",
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AU - Lee, Mary M.

AU - Alberti, K. G M M

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AU - Thorner, Michael O.

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N2 - Serum GH concentrations are increased in fasted or malnourished human subjects. We investigated the dynamic mechanisms underlying this phenomenon in nine normal men by analyzing serum GH concentrations measured in blood obtained at 5-min intervals over 24 h on a control (fed) day and on the second day of a fast with a multiple-parameter deconvolution method to simultaneously resolve endogenous GH secretory and clearance rates. Two days of fasting induced a 5-fold increase in the 24-h endogenous GH production rate [78 ± 12 vs. 371 ± 57 μg/Lv (Lv, liter of distribution volume) or 0.24 ± 0.038 vs. 1.1 ± 0.16 mg/m2 (assuming a distribution volume of 7.9% body weight), P = 0.0001]. This enhanced GH production rate was accounted for by 2-fold increases in the number of GH secretory bursts per 24 h (14 ± 2.3 us. 32 ± 2.4, P = 0.0006) and the mass of GH secreted per burst (6.3 ± 1.2 vs. 11 ± 1.6 μg/Lv, P = 0.002). The latter was a result of increased secretory-event amplitudes (maximal rates of GH release attained within a burst) with unchanged secretory burst durations. GH was secreted in complex volleys composed of multiple discrete secretory bursts. These secretory volleys were separated by shorter intervals of secretory quiescence in the fasted than fed state (respectively, 88 ± 4.2 us. 143 ± 14 min, P = 0.0001). Similarly, within volleys of GH release, constituent individual secretory bursts occurred more frequently during the fast [every 33 ± 0.64 (fasted) vs. every 44 ± 2.0 min (fed), P = 0.0001]. The t1/2 of endogenous GH was not significantly altered by fasting [18 ± 2.2 (fasted) vs. 20 ± 1.5 min (fed), P = 0.47]. Serum insulin-like growth factor I concentrations were unchanged after 56 h of fasting. In conclusion, the present data suggest that starvation-induced enhancement of GH secretion is mediated by an increased frequency of GHRH release, and longer and more pronounced periods of somatostatin withdrawal.

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