Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis

F. Michael Cutrer, Dimos D. Mitsikostas, Gamze Ayata, Margarita Sanchez Del Rio

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We examined the effects of butalbital (30, 100, and 1000 μg/kg) on the number of cells expressing c-fos-like immunoreactivity (c-fos-LI), a marker of neuronal activation, within lamina I, II(o) of the trigeminal nucleus caudalis and the nucleus of the solitary tract 2 hours after the intracisternal injection of capsaicin (0.1 mL; 15.25 mg/mL) or vehicle in urethane-anesthetized guinea pigs (N=45). Robust c-fos-LI was observed within nuclei of cells in the trigeminal nucleus caudalis after capsaicin (329 ± 35). Butalbital dose-dependently reduced the number of labeled cells to a maximum of 66% (1000 μg/kg intraperitoneally [IP], P<.01) in lamina I, II(o) but not within area postrema, medial reticular nucleus, or the nucleus of the solitary tract. Pretreatment with bicuculline (30 μg/kg IP) blocked the effect of butalbital, thereby suggesting the importance of the GABA(A) receptor to activation involved in the transmission of nociceptive information. Our studies suggest the possibility that GABA(A) receptors might provide an important therapeutic target in migraine and related headache disorders.

Original languageEnglish (US)
Pages (from-to)697-704
Number of pages8
JournalHeadache
Volume39
Issue number10
StatePublished - 1999
Externally publishedYes

Fingerprint

Trigeminal Nuclei
Capsaicin
Solitary Nucleus
GABA-A Receptors
Cell Count
Area Postrema
Headache Disorders
Bicuculline
Urethane
Cell Nucleus
Migraine Disorders
Guinea Pigs
Injections
butalbital
Spinal Cord Dorsal Horn
Therapeutics

Keywords

  • c- fos
  • Capsaicin
  • Guinea pig
  • Immunohistochemistry
  • Intracisternal
  • Trigeminal nucleus caudalis

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cutrer, F. M., Mitsikostas, D. D., Ayata, G., & Sanchez Del Rio, M. (1999). Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis. Headache, 39(10), 697-704.

Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis. / Cutrer, F. Michael; Mitsikostas, Dimos D.; Ayata, Gamze; Sanchez Del Rio, Margarita.

In: Headache, Vol. 39, No. 10, 1999, p. 697-704.

Research output: Contribution to journalArticle

Cutrer, FM, Mitsikostas, DD, Ayata, G & Sanchez Del Rio, M 1999, 'Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis', Headache, vol. 39, no. 10, pp. 697-704.
Cutrer FM, Mitsikostas DD, Ayata G, Sanchez Del Rio M. Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis. Headache. 1999;39(10):697-704.
Cutrer, F. Michael ; Mitsikostas, Dimos D. ; Ayata, Gamze ; Sanchez Del Rio, Margarita. / Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis. In: Headache. 1999 ; Vol. 39, No. 10. pp. 697-704.
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