ATP synthesis is coupled to rat liver mitochondrial RNA synthesis

S. Fenella DasGupta, Stanley I. Rapoport, Mariana Gerschenson, Eric Murphy, Gary Fiskum, Stephen J Russell, Krish Chandrasekaran

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Rat liver mitochondria respond to changes in energy demand by modulating the amount of RNA synthesized. Coupled rat liver mitochondria were used to determine the relationship between mitochondrial respiration, ATP levels, and mitochondrial transcription. This system included oxidizable substrates (malate and glutamate) and constituents that could support both mitochondrial respiration and transcription. The respiratory inhibitor rotenone, phosphorylation inhibitor oligomycin, and the uncoupler of oxidative phosphorylation carbonyl-cyanide p-triflouromethoxyphehylhydrazone inhibited RNA synthesis. Addition of ADP stimulated mitochondrial transcription and peak RNA synthesis was observed at 1-2 mM ADP. At ADP concentrations above 2 mM, RNA synthesis decreased. These results demonstrate that mitochondrial transcription is tightly coupled to ATP levels.

Original languageEnglish (US)
Pages (from-to)3-10
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume221
Issue number1-2
DOIs
StatePublished - 2001
Externally publishedYes

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Transcription
Liver
Rats
Adenosine Triphosphate
RNA
Adenosine Diphosphate
Mitochondria
Liver Mitochondrion
Respiration
Oligomycins
Rotenone
Phosphorylation
Oxidative Phosphorylation
Glutamic Acid
mitochondrial RNA
Substrates

Keywords

  • ATP
  • In organello
  • Mitochondria
  • Regulation
  • Respiration
  • Transcription

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Genetics
  • Molecular Biology

Cite this

DasGupta, S. F., Rapoport, S. I., Gerschenson, M., Murphy, E., Fiskum, G., Russell, S. J., & Chandrasekaran, K. (2001). ATP synthesis is coupled to rat liver mitochondrial RNA synthesis. Molecular and Cellular Biochemistry, 221(1-2), 3-10. https://doi.org/10.1023/A:1010812128765

ATP synthesis is coupled to rat liver mitochondrial RNA synthesis. / DasGupta, S. Fenella; Rapoport, Stanley I.; Gerschenson, Mariana; Murphy, Eric; Fiskum, Gary; Russell, Stephen J; Chandrasekaran, Krish.

In: Molecular and Cellular Biochemistry, Vol. 221, No. 1-2, 2001, p. 3-10.

Research output: Contribution to journalArticle

DasGupta, SF, Rapoport, SI, Gerschenson, M, Murphy, E, Fiskum, G, Russell, SJ & Chandrasekaran, K 2001, 'ATP synthesis is coupled to rat liver mitochondrial RNA synthesis', Molecular and Cellular Biochemistry, vol. 221, no. 1-2, pp. 3-10. https://doi.org/10.1023/A:1010812128765
DasGupta, S. Fenella ; Rapoport, Stanley I. ; Gerschenson, Mariana ; Murphy, Eric ; Fiskum, Gary ; Russell, Stephen J ; Chandrasekaran, Krish. / ATP synthesis is coupled to rat liver mitochondrial RNA synthesis. In: Molecular and Cellular Biochemistry. 2001 ; Vol. 221, No. 1-2. pp. 3-10.
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