Ataxia-telangiectasia mutated expression is associated with tobacco smoke exposure in esophageal cancer tissues and benzo[a]pyrene diol epoxide in cell lines

Yan Jiang, Zheng D. Liang, Tsung T. Wu, Liya Cao, Hongfu Zhang, Xiao Chun Xu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Esophageal cancer is a substantial health problem because of its usually late stage at diagnosis and poor prognosis. Tobacco smoking and alcohol use are the most important risk factors in the development of esophageal squamous cell carcinoma (SCC). Our previous study demonstrated the binding of benzo[a]pyrene diol epoxide (BPDE), a carcinogen present in tobacco smoke and environmental pollution, to the ataxia-telangiectasia mutated (ATM) gene. To understand how this binding affects the alteration of ATM expression and to identify biomarkers for the detection of esophageal cancer, we analyzed ATM mRNA expression in tissue specimens from patients with esophageal SCC and premalignant lesions using in situ hybridization. We then performed in vitro experiments to verify and extend our ex vivo observations. We found that ATM expression was increased in esophageal SCC and its premalignant lesions when compared with normal tissues and that increased ATM expression was associated with tobacco smoke exposure and tumor de-differentiation. Moreover, BPDE induced ATM expression in esophageal SCC cell lines in a time-dependent manner. In summary, the BPDE in tobacco smoke may be responsible for increased ATM expression in premalignant and malignant esophageal tissues. Our findings suggest that the ATM gene should be further evaluated as a biomarker for the early detection of esophageal cancer and tobacco use in patients.

Original languageEnglish (US)
Pages (from-to)91-95
Number of pages5
JournalInternational Journal of Cancer
Volume120
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

Fingerprint

Ataxia Telangiectasia
Benzo(a)pyrene
Epoxy Compounds
Esophageal Neoplasms
Smoke
Tobacco
Cell Line
Biomarkers
Tobacco Smoke Pollution
Tobacco Use
Early Detection of Cancer
Carcinogens
Genes
In Situ Hybridization
Smoking
Alcohols
Messenger RNA
Esophageal Squamous Cell Carcinoma
Health

Keywords

  • ATM
  • Biomarker
  • BPDE
  • Esophageal cancer
  • Tobacco smoke

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ataxia-telangiectasia mutated expression is associated with tobacco smoke exposure in esophageal cancer tissues and benzo[a]pyrene diol epoxide in cell lines. / Jiang, Yan; Liang, Zheng D.; Wu, Tsung T.; Cao, Liya; Zhang, Hongfu; Xu, Xiao Chun.

In: International Journal of Cancer, Vol. 120, No. 1, 01.01.2007, p. 91-95.

Research output: Contribution to journalArticle

Jiang, Yan ; Liang, Zheng D. ; Wu, Tsung T. ; Cao, Liya ; Zhang, Hongfu ; Xu, Xiao Chun. / Ataxia-telangiectasia mutated expression is associated with tobacco smoke exposure in esophageal cancer tissues and benzo[a]pyrene diol epoxide in cell lines. In: International Journal of Cancer. 2007 ; Vol. 120, No. 1. pp. 91-95.
@article{d5ba718c31b54453b1a4ef639e2ec9d0,
title = "Ataxia-telangiectasia mutated expression is associated with tobacco smoke exposure in esophageal cancer tissues and benzo[a]pyrene diol epoxide in cell lines",
abstract = "Esophageal cancer is a substantial health problem because of its usually late stage at diagnosis and poor prognosis. Tobacco smoking and alcohol use are the most important risk factors in the development of esophageal squamous cell carcinoma (SCC). Our previous study demonstrated the binding of benzo[a]pyrene diol epoxide (BPDE), a carcinogen present in tobacco smoke and environmental pollution, to the ataxia-telangiectasia mutated (ATM) gene. To understand how this binding affects the alteration of ATM expression and to identify biomarkers for the detection of esophageal cancer, we analyzed ATM mRNA expression in tissue specimens from patients with esophageal SCC and premalignant lesions using in situ hybridization. We then performed in vitro experiments to verify and extend our ex vivo observations. We found that ATM expression was increased in esophageal SCC and its premalignant lesions when compared with normal tissues and that increased ATM expression was associated with tobacco smoke exposure and tumor de-differentiation. Moreover, BPDE induced ATM expression in esophageal SCC cell lines in a time-dependent manner. In summary, the BPDE in tobacco smoke may be responsible for increased ATM expression in premalignant and malignant esophageal tissues. Our findings suggest that the ATM gene should be further evaluated as a biomarker for the early detection of esophageal cancer and tobacco use in patients.",
keywords = "ATM, Biomarker, BPDE, Esophageal cancer, Tobacco smoke",
author = "Yan Jiang and Liang, {Zheng D.} and Wu, {Tsung T.} and Liya Cao and Hongfu Zhang and Xu, {Xiao Chun}",
year = "2007",
month = "1",
day = "1",
doi = "10.1002/ijc.22121",
language = "English (US)",
volume = "120",
pages = "91--95",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Ataxia-telangiectasia mutated expression is associated with tobacco smoke exposure in esophageal cancer tissues and benzo[a]pyrene diol epoxide in cell lines

AU - Jiang, Yan

AU - Liang, Zheng D.

AU - Wu, Tsung T.

AU - Cao, Liya

AU - Zhang, Hongfu

AU - Xu, Xiao Chun

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Esophageal cancer is a substantial health problem because of its usually late stage at diagnosis and poor prognosis. Tobacco smoking and alcohol use are the most important risk factors in the development of esophageal squamous cell carcinoma (SCC). Our previous study demonstrated the binding of benzo[a]pyrene diol epoxide (BPDE), a carcinogen present in tobacco smoke and environmental pollution, to the ataxia-telangiectasia mutated (ATM) gene. To understand how this binding affects the alteration of ATM expression and to identify biomarkers for the detection of esophageal cancer, we analyzed ATM mRNA expression in tissue specimens from patients with esophageal SCC and premalignant lesions using in situ hybridization. We then performed in vitro experiments to verify and extend our ex vivo observations. We found that ATM expression was increased in esophageal SCC and its premalignant lesions when compared with normal tissues and that increased ATM expression was associated with tobacco smoke exposure and tumor de-differentiation. Moreover, BPDE induced ATM expression in esophageal SCC cell lines in a time-dependent manner. In summary, the BPDE in tobacco smoke may be responsible for increased ATM expression in premalignant and malignant esophageal tissues. Our findings suggest that the ATM gene should be further evaluated as a biomarker for the early detection of esophageal cancer and tobacco use in patients.

AB - Esophageal cancer is a substantial health problem because of its usually late stage at diagnosis and poor prognosis. Tobacco smoking and alcohol use are the most important risk factors in the development of esophageal squamous cell carcinoma (SCC). Our previous study demonstrated the binding of benzo[a]pyrene diol epoxide (BPDE), a carcinogen present in tobacco smoke and environmental pollution, to the ataxia-telangiectasia mutated (ATM) gene. To understand how this binding affects the alteration of ATM expression and to identify biomarkers for the detection of esophageal cancer, we analyzed ATM mRNA expression in tissue specimens from patients with esophageal SCC and premalignant lesions using in situ hybridization. We then performed in vitro experiments to verify and extend our ex vivo observations. We found that ATM expression was increased in esophageal SCC and its premalignant lesions when compared with normal tissues and that increased ATM expression was associated with tobacco smoke exposure and tumor de-differentiation. Moreover, BPDE induced ATM expression in esophageal SCC cell lines in a time-dependent manner. In summary, the BPDE in tobacco smoke may be responsible for increased ATM expression in premalignant and malignant esophageal tissues. Our findings suggest that the ATM gene should be further evaluated as a biomarker for the early detection of esophageal cancer and tobacco use in patients.

KW - ATM

KW - Biomarker

KW - BPDE

KW - Esophageal cancer

KW - Tobacco smoke

UR - http://www.scopus.com/inward/record.url?scp=33845404197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845404197&partnerID=8YFLogxK

U2 - 10.1002/ijc.22121

DO - 10.1002/ijc.22121

M3 - Article

C2 - 17019709

AN - SCOPUS:33845404197

VL - 120

SP - 91

EP - 95

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -