Asymmetric endocytosis and remodeling of Β1-integrin adhesions during growth cone chemorepulsion by MAG

Jacob H. Hines, Mohammad Abu-Rub, John R Henley

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Gradients of chemorepellent factors released from myelin may impair axon pathfinding and neuroregeneration after injury. We found that, analogously to the process of chemotaxis in invasive tumor cells, axonal growth cones of Xenopus spinal neurons modulate the functional distribution of integrin receptors during chemorepulsion induced by myelin-associated glycoprotein (MAG). A focal MAG gradient induced polarized endocytosis and concomitant asymmetric loss of Β1-integrin and vinculin-containing adhesions on the repellent side during repulsive turning. Loss of symmetrical Β1-integrin function was both necessary and sufficient for chemorepulsion, which required internalization by clathrin-mediated endocytosis. Induction of repulsive Ca2+ signals was necessary and sufficient for the stimulated rapid endocytosis of Β1-integrin. Altogether, these findings identify Β1-integrin as an important functional cargo during Ca2+-dependent rapid endocytosis stimulated by a diffusible guidance cue. Such dynamic redistribution allows the growth cone to rapidly adjust adhesiveness across its axis, an essential feature for initiating chemotactic turning.

Original languageEnglish (US)
Pages (from-to)829-837
Number of pages9
JournalNature Neuroscience
Volume13
Issue number7
DOIs
StatePublished - Jul 2010

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Myelin-Associated Glycoprotein
Growth Cones
Endocytosis
Integrins
Vinculin
Adhesiveness
Clathrin
Chemotaxis
Myelin Sheath
Xenopus
Cues
Neurons
Wounds and Injuries
Neoplasms

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Asymmetric endocytosis and remodeling of Β1-integrin adhesions during growth cone chemorepulsion by MAG. / Hines, Jacob H.; Abu-Rub, Mohammad; Henley, John R.

In: Nature Neuroscience, Vol. 13, No. 7, 07.2010, p. 829-837.

Research output: Contribution to journalArticle

Hines, Jacob H. ; Abu-Rub, Mohammad ; Henley, John R. / Asymmetric endocytosis and remodeling of Β1-integrin adhesions during growth cone chemorepulsion by MAG. In: Nature Neuroscience. 2010 ; Vol. 13, No. 7. pp. 829-837.
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