A1 Adenosine Receptor Activation Inhibits Inflammation, Necrosis, and Apoptosis after Renal Ischemia-Reperfusion Injury in Mice

H. Thomas Lee, George Gallos, Samih H. Nasr, Charles W. Emala

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135 Citations (Scopus)

Abstract

It was previously demonstrated that preischemic A1 adenosine receptor (AR) activation protects renal function after ischemia-reperfusion (IR) injury in rats. The role of the A1 AR in modulating inflammation, necrosis, and apoptosis in the kidney after IR renal injury was further characterized. C57BL/6 mice were subjected to 30 min of renal ischemia, with or without pretreatment with 1,3-dipropyl-8-cyclopentylxanthine or 2-chlorocyclopentyladenosine (selective A1 AR antagonist and agonist, respectively). Plasma creatinine levels and renal inflammation, necrosis, and apoptosis were compared 24 h after renal injury. C57BL/6 mice that had been pretreated with the A1 AR agonist demonstrated significantly improved renal function and reduced expression of inflammatory markers, necrosis, and apoptosis 24 h after IR injury. In contrast, C57BL/6 mice that had been pretreated with the A1 AR antagonist demonstrated significantly worsened renal function and increased expression of inflammatory markers, necrosis, and apoptosis. In conclusion, it was demonstrated that endogenous and exogenous preischemic activation of the A1 AR protects against IR injury in vivo, through mechanisms that reduce inflammation, necrosis, and apoptosis.

Original languageEnglish (US)
Pages (from-to)102-111
Number of pages10
JournalJournal of the American Society of Nephrology
Volume15
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

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Adenosine A1 Receptors
Reperfusion Injury
Necrosis
Apoptosis
Inflammation
Kidney
Adenosine A1 Receptor Agonists
Adenosine A1 Receptor Antagonists
Inbred C57BL Mouse
Creatinine
Ischemia
Wounds and Injuries

ASJC Scopus subject areas

  • Nephrology

Cite this

A1 Adenosine Receptor Activation Inhibits Inflammation, Necrosis, and Apoptosis after Renal Ischemia-Reperfusion Injury in Mice. / Lee, H. Thomas; Gallos, George; Nasr, Samih H.; Emala, Charles W.

In: Journal of the American Society of Nephrology, Vol. 15, No. 1, 01.2004, p. 102-111.

Research output: Contribution to journalArticle

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