Associations between single nucleotide polymorphisms and haplotypes in cytokine and cytokine receptor genes and immunity to measles vaccination

Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B Kennedy, Robert A. Vierkant, V. Shane Pankratz, Robert M. Jacobson, Gregory A. Poland

Research output: Contribution to journalArticle

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Abstract

Identification of host genetic determinants of measles vaccine-induced immunity can be used to design better vaccines and ultimately predict immune responses to vaccination.We performed a comprehensive candidate gene association study across 801 genetic markers in 56 cytokine/cytokine receptor genes, in a racially diverse cohort of 745 schoolchildren after two doses of MMR vaccine. Using linear regression methodologies we examined associations between SNPs/haplotypes and measles virus-specific immunity.Forty-eight significant SNP associations with variations in neutralizing antibodies and measles-specific IFNγ Elispot responses were identified (p<. 0.05). Our study replicated an important previously found association of a functional IL12B genetic variant rs3212227 with variations in measles-specific humoral immunity (p= 0.037). Similarly, two previously reported promoter IL10 and IL2 polymorphisms (rs1800890 and rs2069762) demonstrated associations with measles-specific cellular immunity in Caucasians (p≤. 0.034). Multiple IL7R polymorphisms, including a non-synonymous functional SNP (rs6897932/Thr244Ile), were associated with humoral (p≤. 0.024) and/or cellular (IFNγ Elispot, p≤. 0.023) measles-specific immune responses in Caucasians, but not African-Americans. Haplotype level analysis confirmed the association of IL7R genetic variants with measles vaccine-induced immunity in the Caucasian group (global p-value = 0.003).Our results validate previous findings and identify new plausible genetic determinants, including IL7R polymorphisms, regulating measles vaccine-induced immunity in a race-specific manner.

Original languageEnglish (US)
Pages (from-to)7883-7895
Number of pages13
JournalVaccine
Volume29
Issue number45
DOIs
StatePublished - Oct 19 2011

Fingerprint

Cytokine Receptors
Measles
Measles Vaccine
Haplotypes
single nucleotide polymorphism
Single Nucleotide Polymorphism
Immunity
haplotypes
Vaccination
cytokines
immunity
vaccination
Cytokines
vaccines
Genes
genetic polymorphism
genes
Measles-Mumps-Rubella Vaccine
Measles virus
immune response

Keywords

  • Cytokine
  • Cytokine receptor
  • Haplotypes
  • Immunity
  • Measles vaccine
  • Single nucleotide polymorphisms

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Associations between single nucleotide polymorphisms and haplotypes in cytokine and cytokine receptor genes and immunity to measles vaccination. / Haralambieva, Iana H.; Ovsyannikova, Inna G.; Kennedy, Richard B; Vierkant, Robert A.; Pankratz, V. Shane; Jacobson, Robert M.; Poland, Gregory A.

In: Vaccine, Vol. 29, No. 45, 19.10.2011, p. 7883-7895.

Research output: Contribution to journalArticle

Haralambieva, Iana H. ; Ovsyannikova, Inna G. ; Kennedy, Richard B ; Vierkant, Robert A. ; Pankratz, V. Shane ; Jacobson, Robert M. ; Poland, Gregory A. / Associations between single nucleotide polymorphisms and haplotypes in cytokine and cytokine receptor genes and immunity to measles vaccination. In: Vaccine. 2011 ; Vol. 29, No. 45. pp. 7883-7895.
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