Association of interleukin-10 promoter polymorphisms with systemic lupus erythematosus

W. P. Chong, Wai Kee Eddie Ip, W. H.S. Wong, C. S. Lau, T. M. Chan, Y. Lung Lau

Research output: Contribution to journalArticle

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Abstract

Several lines of evidence suggest interleukin-10 gene (IL-10) is a candidate gene in susceptibility to systemic lupus erythematosus (SLE). We investigated the association of IL-10 promoter single-nucleotide polymorphisms (SNPs) (-3575T/A, -2849G/A, -2763C/A, -1082A/G, -819T/C and -592A/C) and microsatellites (IL10.R, 1L10.G) with SLE in 554 Hong Kong Chinese patients and 708 ethnically matched controls. Six haplotypes (hts) were identified from the SNPs. The genotype distribution of the ht1 (T-C-A-T-A), which is associated with low IL-10 production, was different in patients and controls (P = 0.009). The homozygous genotype of non-ht1 was significantly increased in patients (P = 0.009, odds ratio (OR) = 1.80, 95% CI: 1.15-2.82). The frequency of IL10.G4 of IL10.G was also significantly increased in patients (P = 0.017, OR = 2.53, 95% CI: 1.18-5.40). We found that the homozygous non-ht1 combined with short allele (CA repeat number ≤21) of 1L10.G has a dose-dependent effect on SLE susceptibility: non-ht1/non-ht1 with homozygous short allele showed a higher OR (OR = 4.11, 95% CI: 1.27-13.2, P = 0.018) of association with SLE than the genotype of non-ht1/non-ht1 with heterozygous short/long allele (OR = 2.98, 95% CI: 1.26-7.07, P = 0.013) and homozygous long allele (OR = 1.05, 95% CI: 0.62-1.78, P = 0.848). The frequency of non-ht1 was significantly increased in patients with serositis (P < 0.0001, OR = 2.42, 95% CI: 1.55-3.80). In conclusion, the high expression promoter genotype is associated with SLE in Chinese.

Original languageEnglish (US)
Pages (from-to)484-492
Number of pages9
JournalGenes and Immunity
Volume5
Issue number6
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Fingerprint

Systemic Lupus Erythematosus
Interleukin-10
Odds Ratio
Alleles
Genotype
Genes
Single Nucleotide Polymorphism
Serositis
Hong Kong
Microsatellite Repeats
Haplotypes

Keywords

  • Haplotype
  • Interleukin 10
  • Polymorphism
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Genetics(clinical)

Cite this

Association of interleukin-10 promoter polymorphisms with systemic lupus erythematosus. / Chong, W. P.; Ip, Wai Kee Eddie; Wong, W. H.S.; Lau, C. S.; Chan, T. M.; Lau, Y. Lung.

In: Genes and Immunity, Vol. 5, No. 6, 01.09.2004, p. 484-492.

Research output: Contribution to journalArticle

Chong, W. P. ; Ip, Wai Kee Eddie ; Wong, W. H.S. ; Lau, C. S. ; Chan, T. M. ; Lau, Y. Lung. / Association of interleukin-10 promoter polymorphisms with systemic lupus erythematosus. In: Genes and Immunity. 2004 ; Vol. 5, No. 6. pp. 484-492.
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abstract = "Several lines of evidence suggest interleukin-10 gene (IL-10) is a candidate gene in susceptibility to systemic lupus erythematosus (SLE). We investigated the association of IL-10 promoter single-nucleotide polymorphisms (SNPs) (-3575T/A, -2849G/A, -2763C/A, -1082A/G, -819T/C and -592A/C) and microsatellites (IL10.R, 1L10.G) with SLE in 554 Hong Kong Chinese patients and 708 ethnically matched controls. Six haplotypes (hts) were identified from the SNPs. The genotype distribution of the ht1 (T-C-A-T-A), which is associated with low IL-10 production, was different in patients and controls (P = 0.009). The homozygous genotype of non-ht1 was significantly increased in patients (P = 0.009, odds ratio (OR) = 1.80, 95{\%} CI: 1.15-2.82). The frequency of IL10.G4 of IL10.G was also significantly increased in patients (P = 0.017, OR = 2.53, 95{\%} CI: 1.18-5.40). We found that the homozygous non-ht1 combined with short allele (CA repeat number ≤21) of 1L10.G has a dose-dependent effect on SLE susceptibility: non-ht1/non-ht1 with homozygous short allele showed a higher OR (OR = 4.11, 95{\%} CI: 1.27-13.2, P = 0.018) of association with SLE than the genotype of non-ht1/non-ht1 with heterozygous short/long allele (OR = 2.98, 95{\%} CI: 1.26-7.07, P = 0.013) and homozygous long allele (OR = 1.05, 95{\%} CI: 0.62-1.78, P = 0.848). The frequency of non-ht1 was significantly increased in patients with serositis (P < 0.0001, OR = 2.42, 95{\%} CI: 1.55-3.80). In conclusion, the high expression promoter genotype is associated with SLE in Chinese.",
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T1 - Association of interleukin-10 promoter polymorphisms with systemic lupus erythematosus

AU - Chong, W. P.

AU - Ip, Wai Kee Eddie

AU - Wong, W. H.S.

AU - Lau, C. S.

AU - Chan, T. M.

AU - Lau, Y. Lung

PY - 2004/9/1

Y1 - 2004/9/1

N2 - Several lines of evidence suggest interleukin-10 gene (IL-10) is a candidate gene in susceptibility to systemic lupus erythematosus (SLE). We investigated the association of IL-10 promoter single-nucleotide polymorphisms (SNPs) (-3575T/A, -2849G/A, -2763C/A, -1082A/G, -819T/C and -592A/C) and microsatellites (IL10.R, 1L10.G) with SLE in 554 Hong Kong Chinese patients and 708 ethnically matched controls. Six haplotypes (hts) were identified from the SNPs. The genotype distribution of the ht1 (T-C-A-T-A), which is associated with low IL-10 production, was different in patients and controls (P = 0.009). The homozygous genotype of non-ht1 was significantly increased in patients (P = 0.009, odds ratio (OR) = 1.80, 95% CI: 1.15-2.82). The frequency of IL10.G4 of IL10.G was also significantly increased in patients (P = 0.017, OR = 2.53, 95% CI: 1.18-5.40). We found that the homozygous non-ht1 combined with short allele (CA repeat number ≤21) of 1L10.G has a dose-dependent effect on SLE susceptibility: non-ht1/non-ht1 with homozygous short allele showed a higher OR (OR = 4.11, 95% CI: 1.27-13.2, P = 0.018) of association with SLE than the genotype of non-ht1/non-ht1 with heterozygous short/long allele (OR = 2.98, 95% CI: 1.26-7.07, P = 0.013) and homozygous long allele (OR = 1.05, 95% CI: 0.62-1.78, P = 0.848). The frequency of non-ht1 was significantly increased in patients with serositis (P < 0.0001, OR = 2.42, 95% CI: 1.55-3.80). In conclusion, the high expression promoter genotype is associated with SLE in Chinese.

AB - Several lines of evidence suggest interleukin-10 gene (IL-10) is a candidate gene in susceptibility to systemic lupus erythematosus (SLE). We investigated the association of IL-10 promoter single-nucleotide polymorphisms (SNPs) (-3575T/A, -2849G/A, -2763C/A, -1082A/G, -819T/C and -592A/C) and microsatellites (IL10.R, 1L10.G) with SLE in 554 Hong Kong Chinese patients and 708 ethnically matched controls. Six haplotypes (hts) were identified from the SNPs. The genotype distribution of the ht1 (T-C-A-T-A), which is associated with low IL-10 production, was different in patients and controls (P = 0.009). The homozygous genotype of non-ht1 was significantly increased in patients (P = 0.009, odds ratio (OR) = 1.80, 95% CI: 1.15-2.82). The frequency of IL10.G4 of IL10.G was also significantly increased in patients (P = 0.017, OR = 2.53, 95% CI: 1.18-5.40). We found that the homozygous non-ht1 combined with short allele (CA repeat number ≤21) of 1L10.G has a dose-dependent effect on SLE susceptibility: non-ht1/non-ht1 with homozygous short allele showed a higher OR (OR = 4.11, 95% CI: 1.27-13.2, P = 0.018) of association with SLE than the genotype of non-ht1/non-ht1 with heterozygous short/long allele (OR = 2.98, 95% CI: 1.26-7.07, P = 0.013) and homozygous long allele (OR = 1.05, 95% CI: 0.62-1.78, P = 0.848). The frequency of non-ht1 was significantly increased in patients with serositis (P < 0.0001, OR = 2.42, 95% CI: 1.55-3.80). In conclusion, the high expression promoter genotype is associated with SLE in Chinese.

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