Association of bile acid receptor TGR5 variation and transit in health and lower functional gastrointestinal disorders

Michael Camilleri, Maria I Vazquez Roque, P. Carlson, D. Burton, B. S. Wong, A. R. Zinsmeister

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background The membrane bound bile acid (BA) receptor, TGR5, is located on myenteric, cholinergic and nitrergic neurons in colon and proximal small intestine. Our aim was to assess the association of genetic variation in TGR5 and small bowel transit (SBT) and colonic transit. Methods In 230 healthy controls and 414 patients with lower functional GI disorders [FGID: irritable bowel syndrome (IBS)-alternators (Alt) 84, IBS-constipation (IBS-C) 157, IBS-diarrhea (IBS-D) 173], we tested the association between TGR5 SNP rs11554825 (minor allele frequency 41%) with symptom phenotype (total cohort) and intermediate phenotype (SBT or colonic transit by radioscintigraphy) which was available in 213 people in this cohort. The association with symptom phenotype was assessed using logistic regression, while the association with colonic filling at 6h (CF6), and colonic transit [geometric center (GC) at 24h] was assessed using ancova, in each instance assuming a dominant genetic model. Key Results There was no significant association with symptom phenotype. We observed a potential association of SNP rs11554825 with overall transit: CF6 (P=0.061) and GC24 (P=0.083). The association of the SNP with CF6 in the IBS-D subgroup (P=0.017) indicated the TC/CC subgroup had an average 50% faster SBT compared with the TT subgroup. In IBS-D patients, GC24 was not significantly associated with rs11554825 (TC/CC vs TT). Conclusions & Inferences Variation in TGR5 may contribute to altered SBT and colonic transit in lower FGID. Further studies are required to characterize the potential role of BA receptor, TGR5, in the mechanism and treatment of bowel dysfunction in lower FGID.

Original languageEnglish (US)
Pages (from-to)995
Number of pages1
JournalNeurogastroenterology and Motility
Volume23
Issue number11
DOIs
StatePublished - Nov 2011

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Irritable Bowel Syndrome
Gastrointestinal Diseases
Bile Acids and Salts
Health
Single Nucleotide Polymorphism
Phenotype
Nitrergic Neurons
Cholinergic Neurons
Genetic Models
Constipation
Gene Frequency
Small Intestine
Diarrhea
Colon
Logistic Models
Membranes

Keywords

  • Colon
  • Diarrhea
  • GpBAR1
  • Intestine

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Association of bile acid receptor TGR5 variation and transit in health and lower functional gastrointestinal disorders. / Camilleri, Michael; Vazquez Roque, Maria I; Carlson, P.; Burton, D.; Wong, B. S.; Zinsmeister, A. R.

In: Neurogastroenterology and Motility, Vol. 23, No. 11, 11.2011, p. 995.

Research output: Contribution to journalArticle

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abstract = "Background The membrane bound bile acid (BA) receptor, TGR5, is located on myenteric, cholinergic and nitrergic neurons in colon and proximal small intestine. Our aim was to assess the association of genetic variation in TGR5 and small bowel transit (SBT) and colonic transit. Methods In 230 healthy controls and 414 patients with lower functional GI disorders [FGID: irritable bowel syndrome (IBS)-alternators (Alt) 84, IBS-constipation (IBS-C) 157, IBS-diarrhea (IBS-D) 173], we tested the association between TGR5 SNP rs11554825 (minor allele frequency 41{\%}) with symptom phenotype (total cohort) and intermediate phenotype (SBT or colonic transit by radioscintigraphy) which was available in 213 people in this cohort. The association with symptom phenotype was assessed using logistic regression, while the association with colonic filling at 6h (CF6), and colonic transit [geometric center (GC) at 24h] was assessed using ancova, in each instance assuming a dominant genetic model. Key Results There was no significant association with symptom phenotype. We observed a potential association of SNP rs11554825 with overall transit: CF6 (P=0.061) and GC24 (P=0.083). The association of the SNP with CF6 in the IBS-D subgroup (P=0.017) indicated the TC/CC subgroup had an average 50{\%} faster SBT compared with the TT subgroup. In IBS-D patients, GC24 was not significantly associated with rs11554825 (TC/CC vs TT). Conclusions & Inferences Variation in TGR5 may contribute to altered SBT and colonic transit in lower FGID. Further studies are required to characterize the potential role of BA receptor, TGR5, in the mechanism and treatment of bowel dysfunction in lower FGID.",
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