Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line

T. E. Gribbin, C. K. Stein, J. S. Harrison, T. W. Glover, C. A. Hanson, J. J. Wasmuth, R. L. Cody, B. S. Mitchell

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have found a single 4p+ chromosomal abnormality, 46,XX, -4,+der(4)t(3;4)(q13.3;p16), in a patient with an unusual B cell leukemia of mature phenotype characterized by a high white cell count, tartrate-resistant acid phosphatase-positive malignant cells, splenic white pulp proliferation, and a serum IgM monoclonal gammopathy. The malignant cells were characterized by surface expression of CD19(B4), CD20 (B1), IgM, IgD, kappa, and HLA-DR. They were weakly positive for CDE21 (B2) and negative for CD25 (interleukin-2 receptor). The malignant cells also showed clonal rearrangement of the immunoglobulin heavy chain and kappa light chain genes. A cell line, designated HCLW-3B, was derived from unstimulated peripheral blood obtained during the leukemic phase and was found to contain the same 4p+ chromosomal abnormality as well as genomic sequences of Epstein-Barr virus nuclear antigen. A somatic cell hybrid constructed from HCLW-3B containing the derivative chromosome 4 was used to confirm that chromosome 3q was the source of the translocated material. The availability of a cell line which is clonally derived from the patient's circulating leukemia cells should permit further characterization of his translocation at the molecular level.

Original languageEnglish (US)
Pages (from-to)643-647
Number of pages5
JournalLeukemia
Volume3
Issue number9
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Gribbin, T. E., Stein, C. K., Harrison, J. S., Glover, T. W., Hanson, C. A., Wasmuth, J. J., Cody, R. L., & Mitchell, B. S. (1989). Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line. Leukemia, 3(9), 643-647.