Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line

T. E. Gribbin, C. K. Stein, J. S. Harrison, T. W. Glover, C. A. Hanson, J. J. Wasmuth, R. L. Cody, B. S. Mitchell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have found a single 4p+ chromosomal abnormality, 46,XX, -4,+der(4)t(3;4)(q13.3;p16), in a patient with an unusual B cell leukemia of mature phenotype characterized by a high white cell count, tartrate-resistant acid phosphatase-positive malignant cells, splenic white pulp proliferation, and a serum IgM monoclonal gammopathy. The malignant cells were characterized by surface expression of CD19(B4), CD20 (B1), IgM, IgD, kappa, and HLA-DR. They were weakly positive for CDE21 (B2) and negative for CD25 (interleukin-2 receptor). The malignant cells also showed clonal rearrangement of the immunoglobulin heavy chain and kappa light chain genes. A cell line, designated HCLW-3B, was derived from unstimulated peripheral blood obtained during the leukemic phase and was found to contain the same 4p+ chromosomal abnormality as well as genomic sequences of Epstein-Barr virus nuclear antigen. A somatic cell hybrid constructed from HCLW-3B containing the derivative chromosome 4 was used to confirm that chromosome 3q was the source of the translocated material. The availability of a cell line which is clonally derived from the patient's circulating leukemia cells should permit further characterization of his translocation at the molecular level.

Original languageEnglish (US)
Pages (from-to)643-647
Number of pages5
JournalLeukemia
Volume3
Issue number9
StatePublished - 1989
Externally publishedYes

Fingerprint

B-Cell Leukemia
Chromosome Aberrations
Cell Line
Immunoglobulin M
Epstein-Barr Virus Nuclear Antigens
Immunoglobulin D
Immunoglobulin Heavy Chains
Chromosomes, Human, Pair 4
Paraproteinemias
Hybrid Cells
Interleukin-2 Receptors
HLA-DR Antigens
Leukemia
Cell Count
Chromosomes
Phenotype
Light
Serum
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Gribbin, T. E., Stein, C. K., Harrison, J. S., Glover, T. W., Hanson, C. A., Wasmuth, J. J., ... Mitchell, B. S. (1989). Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line. Leukemia, 3(9), 643-647.

Association of a mature B cell leukemia with a 4p+ chromosomal abnormality : Derivation and characterization of a cell line. / Gribbin, T. E.; Stein, C. K.; Harrison, J. S.; Glover, T. W.; Hanson, C. A.; Wasmuth, J. J.; Cody, R. L.; Mitchell, B. S.

In: Leukemia, Vol. 3, No. 9, 1989, p. 643-647.

Research output: Contribution to journalArticle

Gribbin, TE, Stein, CK, Harrison, JS, Glover, TW, Hanson, CA, Wasmuth, JJ, Cody, RL & Mitchell, BS 1989, 'Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line', Leukemia, vol. 3, no. 9, pp. 643-647.
Gribbin TE, Stein CK, Harrison JS, Glover TW, Hanson CA, Wasmuth JJ et al. Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: Derivation and characterization of a cell line. Leukemia. 1989;3(9):643-647.
Gribbin, T. E. ; Stein, C. K. ; Harrison, J. S. ; Glover, T. W. ; Hanson, C. A. ; Wasmuth, J. J. ; Cody, R. L. ; Mitchell, B. S. / Association of a mature B cell leukemia with a 4p+ chromosomal abnormality : Derivation and characterization of a cell line. In: Leukemia. 1989 ; Vol. 3, No. 9. pp. 643-647.
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