TY - JOUR
T1 - Association of a mature B cell leukemia with a 4p+ chromosomal abnormality
T2 - Derivation and characterization of a cell line
AU - Gribbin, T. E.
AU - Stein, C. K.
AU - Harrison, J. S.
AU - Glover, T. W.
AU - Hanson, C. A.
AU - Wasmuth, J. J.
AU - Cody, R. L.
AU - Mitchell, B. S.
PY - 1989
Y1 - 1989
N2 - We have found a single 4p+ chromosomal abnormality, 46,XX, -4,+der(4)t(3;4)(q13.3;p16), in a patient with an unusual B cell leukemia of mature phenotype characterized by a high white cell count, tartrate-resistant acid phosphatase-positive malignant cells, splenic white pulp proliferation, and a serum IgM monoclonal gammopathy. The malignant cells were characterized by surface expression of CD19(B4), CD20 (B1), IgM, IgD, kappa, and HLA-DR. They were weakly positive for CDE21 (B2) and negative for CD25 (interleukin-2 receptor). The malignant cells also showed clonal rearrangement of the immunoglobulin heavy chain and kappa light chain genes. A cell line, designated HCLW-3B, was derived from unstimulated peripheral blood obtained during the leukemic phase and was found to contain the same 4p+ chromosomal abnormality as well as genomic sequences of Epstein-Barr virus nuclear antigen. A somatic cell hybrid constructed from HCLW-3B containing the derivative chromosome 4 was used to confirm that chromosome 3q was the source of the translocated material. The availability of a cell line which is clonally derived from the patient's circulating leukemia cells should permit further characterization of his translocation at the molecular level.
AB - We have found a single 4p+ chromosomal abnormality, 46,XX, -4,+der(4)t(3;4)(q13.3;p16), in a patient with an unusual B cell leukemia of mature phenotype characterized by a high white cell count, tartrate-resistant acid phosphatase-positive malignant cells, splenic white pulp proliferation, and a serum IgM monoclonal gammopathy. The malignant cells were characterized by surface expression of CD19(B4), CD20 (B1), IgM, IgD, kappa, and HLA-DR. They were weakly positive for CDE21 (B2) and negative for CD25 (interleukin-2 receptor). The malignant cells also showed clonal rearrangement of the immunoglobulin heavy chain and kappa light chain genes. A cell line, designated HCLW-3B, was derived from unstimulated peripheral blood obtained during the leukemic phase and was found to contain the same 4p+ chromosomal abnormality as well as genomic sequences of Epstein-Barr virus nuclear antigen. A somatic cell hybrid constructed from HCLW-3B containing the derivative chromosome 4 was used to confirm that chromosome 3q was the source of the translocated material. The availability of a cell line which is clonally derived from the patient's circulating leukemia cells should permit further characterization of his translocation at the molecular level.
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M3 - Article
C2 - 2548046
AN - SCOPUS:0024431780
SN - 0887-6924
VL - 3
SP - 643
EP - 647
JO - Leukemia
JF - Leukemia
IS - 9
ER -