Association between NEDD4L gene and sodium lithium countertransport

Xiaojing Zheng, Alanna C. Morrison, Eleanor Feingold, Stephen T. Turner, Robert E. Ferrell

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

BackgroundSodium lithium countertransport (SLC) is an intermediate phenotype of essential hypertension. The aim of this study was to identify candidate genes for SLC by a strategy of combining gene expression profiling and linkage analysis, and to examine the association between the candidate gene and SLC as well as hypertension.MethodsIn order to identify SLC-related genes, the top 1% of the genes that were differentially expressed between high- and low-SLC groups in a gene expression microarray were compared with published SLC/hypertension gene linkage maps so as to identify regions of overlap. The association between the genetic variation in the candidate gene and the SLC and blood pressure phenotypes were further assessed in the Rochester Family Heart Study (RFHS) involving 1,815 individuals of European ancestry, belonging to 252 pedigrees.ResultsBased on gene expression profiling and evidence from genome-wide linkage analysis, and in the light of its potential biochemical function, E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) was identified as being both a positional and a functional candidate gene for SLC. The difference in expressions of NEDD4L between the high- and low-SLC groups was confirmed by reverse transcription-PCR (RT-PCR) analysis. After adjusting for age, sex, and body mass index (BMI), four single-nucleotide polymorphisms (SNPs) in NEDD4L were found to be associated with SLC (P< 0.05). Further, haplotype analysis revealed that, after correction for multiple testing, one of the haplotypes (H2) was still significantly (P = 0.006) associated with SLC.ConclusionsThe strategy of combining gene expression profiling and linkage analysis successfully guided us in identifying NEDD4L as a candidate gene involved in regulating SLC activity. Variations in the NEDD4L gene are associated with SLC activity.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalAmerican journal of hypertension
Volume24
Issue number2
DOIs
StatePublished - Feb 2011

Keywords

  • NEDD4L
  • blood pressure
  • genetic association
  • hypertension
  • sodium lithium countertransport

ASJC Scopus subject areas

  • Internal Medicine

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