Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

Jeanette Erdmann; Daphne Shimron-Abarbanell; Viji Shridhar; David I. Smith; Peter Propping; Markus M. Nöthen

doi:10.3109/09687689709048173

Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

Jeanette Erdmann, Daphne Shimron-Abarbanell, Viji Shridhar, David I. Smith, Peter Propping, Markus M. Nöthen

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In the present study, we report the chromosomal localization of the human 5-HT(1F) receptor gene (HTR1F) by the analysis of somatic cell hybrids. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived from the relatively non-conserved 5'-untranslated and coding region. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR1F to human chromosome 3. To confirm the localization on chromosome 3 and to further sublocalize the HTR1F gene, a set of human cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HTR1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.

Original language	English (US)
Pages (from-to)	133-135
Number of pages	3
Journal	Molecular Membrane Biology
Volume	14
Issue number	3
DOIs	https://doi.org/10.3109/09687689709048173
State	Published - 1997

Keywords

5-HT(1F)
Chromosomal localization
Human chromosome 3,5-hydroxytryptamine
Serotonin

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.3109/09687689709048173

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title = "Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)",

abstract = "In the present study, we report the chromosomal localization of the human 5-HT(1F) receptor gene (HTR1F) by the analysis of somatic cell hybrids. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived from the relatively non-conserved 5'-untranslated and coding region. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR1F to human chromosome 3. To confirm the localization on chromosome 3 and to further sublocalize the HTR1F gene, a set of human cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HTR1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.",

keywords = "5-HT(1F), Chromosomal localization, Human chromosome 3,5-hydroxytryptamine, Serotonin",

author = "Jeanette Erdmann and Daphne Shimron-Abarbanell and Viji Shridhar and Smith, {David I.} and Peter Propping and N{\"o}then, {Markus M.}",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (SFB 400 'Molekulare Grundlagen zentralnervoser Erkrankungen', Teilprojekt A3).",

year = "1997",

doi = "10.3109/09687689709048173",

language = "English (US)",

volume = "14",

pages = "133--135",

journal = "Membrane Biochemistry",

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T1 - Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

AU - Erdmann, Jeanette

AU - Shimron-Abarbanell, Daphne

AU - Shridhar, Viji

AU - Smith, David I.

AU - Propping, Peter

AU - Nöthen, Markus M.

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (SFB 400 'Molekulare Grundlagen zentralnervoser Erkrankungen', Teilprojekt A3).

PY - 1997

Y1 - 1997

N2 - In the present study, we report the chromosomal localization of the human 5-HT(1F) receptor gene (HTR1F) by the analysis of somatic cell hybrids. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived from the relatively non-conserved 5'-untranslated and coding region. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR1F to human chromosome 3. To confirm the localization on chromosome 3 and to further sublocalize the HTR1F gene, a set of human cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HTR1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.

AB - In the present study, we report the chromosomal localization of the human 5-HT(1F) receptor gene (HTR1F) by the analysis of somatic cell hybrids. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived from the relatively non-conserved 5'-untranslated and coding region. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR1F to human chromosome 3. To confirm the localization on chromosome 3 and to further sublocalize the HTR1F gene, a set of human cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HTR1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.

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KW - Serotonin

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Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

Abstract

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