Assessment of Renal Osteodystrophy via Computational Analysis of Label-free Raman Detection of Multiple Biomarkers

Marian Manciu, Mario Cardenas, Kevin E. Bennet, Avudaiappan Maran, Michael J. Yaszemski, Theresa A. Maldonado, Diana Magiricu, Felicia S. Manciu

Research output: Contribution to journalArticle

Abstract

Accurate clinical evaluation of renal osteodystrophy (ROD) is currently accomplished using invasive in vivo transiliac bone biopsy, followed by in vitro histomorphometry. In this study, we demonstrate that an alternative method for ROD assessment is through a fast, label-free Raman recording of multiple biomarkers combined with computational analysis for predicting the minimally required number of spectra for sample classification at defined accuracies. Four clinically relevant biomarkers: the mineral-to-matrix ratio, the carbonate-to-matrix ratio, phenylalanine, and calcium contents were experimentally determined and simultaneously considered as input to a linear discriminant analysis (LDA). Additionally, sample evaluation was performed with a linear support vector machine (LSVM) algorithm, with a 300 variable input. The computed probabilities based on a single spectrum were only marginally different (~80% from LDA and ~87% from LSVM), both providing an unacceptable classification power for a correct sample assignment. However, the Type I and Type II assignment errors confirm that a relatively small number of independent spectra (7 spectra for Type I and 5 spectra for Type II) is necessary for a p < 0.05 error probability. This low number of spectra supports the practicality of future in vivo Raman translation for a fast and accurate ROD detection in clinical settings.

Original languageEnglish (US)
Article number79
JournalDiagnostics
Volume10
Issue number2
DOIs
StatePublished - Jan 1 2020

Keywords

  • Artificial intelligence
  • Diagnostic devices
  • Label-free detection
  • Multiple biomarkers
  • Raman spectroscopy
  • Renal osteodystrophy
  • Statistical analysis

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Manciu, M., Cardenas, M., Bennet, K. E., Maran, A., Yaszemski, M. J., Maldonado, T. A., Magiricu, D., & Manciu, F. S. (2020). Assessment of Renal Osteodystrophy via Computational Analysis of Label-free Raman Detection of Multiple Biomarkers. Diagnostics, 10(2), [79]. https://doi.org/10.3390/diagnostics10020079