Assessment of fluorescence in situ hybridization and hybrid capture 2 analyses of cervical cytology specimens diagnosed as low grade squamous intraepithelial lesion for the detection of high grade cervical intraepithelial neoplasia

Jesse S. Voss, Benjamin R. Kipp, Michael B. Campion, Irina A. Sokolova, Michael R. Henry, Kevin C. Halling, Amy C. Clayton

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

OBJECTIVE: To assess Hybrid Capture 2 (HC2) and fluorescence in situ hybridization (FISH) for the detection of cervical intraepithelial neoplasia 2 or worse (CIN 2+) in patients with a cytologic diagnosis of low grade squamous intraepithelial lesion (LSIL). STUDY DESIGN: Residual samples from 115 LSIL-diagnosed cervical cytology specimens were evaluated by high-risk human papillomavirus (HR-HPV) HC2 testing and FISH using biotin-labeled probes to HR-HPV and chromosomal probes to 3q26 (TERC) and 8q24 (CMYC). A cervical biopsy diagnosis of CIN 2+ was considered as evidence of high grade disease. RESULTS: The positive and negative predictive values of HC2 and FISH for detecting patients with CIN 2+ were 32% vs. 37% and 100% vs. 93%, respectively. The sensitivities of HC2 and FISH for CIN 2+ were not significantly different (100% vs. 90%, p=0.25), while the specificity of HC2 was significantly lower than that of FISH (28% vs. 48%, p=0.003). FISH diagnosed fewer specimens as positive as compared to HC2 (62% vs. 79%). CONCLUSION: These preliminary data suggest that FISH testing may be useful for determining which patients with LSIL are most likely to have CIN 2+ on clinical follow-up.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalAnalytical and Quantitative Cytology and Histology
Volume32
Issue number3
StatePublished - Jun 2010

Keywords

  • Biomarkers
  • Cervical diseases
  • Hybrid capture
  • In situ hybridization, fluorescence

ASJC Scopus subject areas

  • Anatomy
  • Histology

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