Assessment of endothelialization of aneurysm wall over time in a rabbit model through CD31 scoring

Praveen Kolumam Parameswaran, Daying Dai, Yong Hong Ding, Tina Gunderson, David F. Kallmes, Ramanathan Kadirvel

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background Intracranial aneurysms represent a significant health concern and are poorly understood despite decades of research. Our study focused on understanding temporal patterns of endothelial cell distribution in different spatial locations within the aneurysm early after creation in a rabbit model. Methods Elastase induced saccular aneurysms were created in rabbits and harvested on day 1 (n=3) and after 2 (n=5), 4 (n=4), 8 (n=5), and 12 (n=6) weeks. Sham operated controls (n=3) were harvested on the same day. Aneurysm and control tissue samples were subjected to en face whole mount CD31 staining for endothelial cells. Semiquantitative scoring was performed on the basis of endothelial coverage of the vessel wall (proximal, middle, and distal portions of the aneurysm dome). Mixed effects models were used to assess the effect of time and aneurysm section on endothelial coverage. Results Aneurysmal segments were near completely de-endothelialized at 4 and 8 weeks but had re-endothelialized by 12 weeks. Compared with controls, aneurysms at all time points showed decreased endothelialization, but the difference was only significant compared with the 4 and 8 week groups. Both time (P=0.03) and aneurysm section (P=0.07) were significantly associated with the degree of endothelialization. Proximal locations showed increased endothelialization compared with distal locations (P=0.03). Conclusion In experimental aneurysms of rabbits, endothelial cells regress during the first month after creation, followed by ascending re-endothelialization that stays incomplete. These findings suggest that re-population of endothelial cells comes from resident cells in the adjacent parent artery and that deranged hemodynamics may affect full reconstitution of endothelial cells long term.

Original languageEnglish (US)
Pages (from-to)888-891
Number of pages4
JournalJournal of neurointerventional surgery
Volume10
Issue number9
DOIs
StatePublished - Sep 2018

Keywords

  • aneurysm
  • vessel wall

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Assessment of endothelialization of aneurysm wall over time in a rabbit model through CD31 scoring'. Together they form a unique fingerprint.

  • Cite this