Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis

Megan L. Krause, John Manley III Davis, Keith L Knutson, Michael A. Strausbach, Cynthia Crowson, Terry M Therneau, Peter J. Wettstein, Eric Lawrence Matteson, Sherine E. Gabriel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalClinical Immunology
Volume141
Issue number1
DOIs
StatePublished - Oct 2011

Fingerprint

Rheumatoid Arthritis
Leukocytes
Cytokines
Infection
Biomarkers
Interleukin-17
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Interleukin-10
Interleukin-2
Medical Records
Longitudinal Studies
Blood Cells
Prospective Studies
Therapeutics

Keywords

  • Immune response
  • Immune signature
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis. / Krause, Megan L.; Davis, John Manley III; Knutson, Keith L; Strausbach, Michael A.; Crowson, Cynthia; Therneau, Terry M; Wettstein, Peter J.; Matteson, Eric Lawrence; Gabriel, Sherine E.

In: Clinical Immunology, Vol. 141, No. 1, 10.2011, p. 67-72.

Research output: Contribution to journalArticle

@article{adc49a484f8b4c74b130ba2f282f9931,
title = "Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis",
abstract = "Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.",
keywords = "Immune response, Immune signature, Peripheral blood mononuclear cells",
author = "Krause, {Megan L.} and Davis, {John Manley III} and Knutson, {Keith L} and Strausbach, {Michael A.} and Cynthia Crowson and Therneau, {Terry M} and Wettstein, {Peter J.} and Matteson, {Eric Lawrence} and Gabriel, {Sherine E.}",
year = "2011",
month = "10",
doi = "10.1016/j.clim.2011.05.008",
language = "English (US)",
volume = "141",
pages = "67--72",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis

AU - Krause, Megan L.

AU - Davis, John Manley III

AU - Knutson, Keith L

AU - Strausbach, Michael A.

AU - Crowson, Cynthia

AU - Therneau, Terry M

AU - Wettstein, Peter J.

AU - Matteson, Eric Lawrence

AU - Gabriel, Sherine E.

PY - 2011/10

Y1 - 2011/10

N2 - Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.

AB - Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.

KW - Immune response

KW - Immune signature

KW - Peripheral blood mononuclear cells

UR - http://www.scopus.com/inward/record.url?scp=80053131239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053131239&partnerID=8YFLogxK

U2 - 10.1016/j.clim.2011.05.008

DO - 10.1016/j.clim.2011.05.008

M3 - Article

C2 - 21703930

AN - SCOPUS:80053131239

VL - 141

SP - 67

EP - 72

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 1

ER -