Assembly of the AAA ATPase Vps4 on ESCRT-III

Anna Shestakova, Abraham Hanono, Stacey Drosner, Matt Curtiss, Brian A. Davies, David J Katzmann, Markus Babst

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Vps4 is a key enzyme that functions in endosomal protein trafficking, cytokinesis, and retroviral budding. Vps4 activity is regulated by its recruitment from the cytoplasm to ESCRT-III, where the protein oligomerizes into an active ATPase. The recruitment and oligomerization steps are mediated by a complex network of at least 12 distinct interactions between Vps4, ESCRT-III, Ist1, Vta1, and Did2. The order of events leading to active, ESCRT-III-associated Vps4 is poorly understood. In this study we present a systematic in vivo analysis of the Vps4 interaction network. The data demonstrated a high degree of redundancy in the network. Although no single interaction was found to be essential for the localization or activity of Vps4, certain interactions proved more important than others. The most significant among these were the binding of Vps4 to Vta1 and to the ESCRT-III subunits Vps2 and Snf7. In our model we propose the formation of a recruitment complex in the cytoplasm that is composed of Did2-Ist1-Vps4, which upon binding to ESCRT-III recruits Vta1. Vta1 in turn is predicted to cause a rearrangement of the Vps4 interactions that initiates the assembly of the active Vps4 oligomer.

Original languageEnglish (US)
Pages (from-to)1059-1071
Number of pages13
JournalMolecular Biology of the Cell
Volume21
Issue number6
DOIs
StatePublished - Mar 15 2010

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Endosomal Sorting Complexes Required for Transport
Adenosine Triphosphatases
Cytoplasm
Cytokinesis
Protein Transport
Enzymes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Shestakova, A., Hanono, A., Drosner, S., Curtiss, M., Davies, B. A., Katzmann, D. J., & Babst, M. (2010). Assembly of the AAA ATPase Vps4 on ESCRT-III. Molecular Biology of the Cell, 21(6), 1059-1071. https://doi.org/10.1091/mbc.E09-07-0572

Assembly of the AAA ATPase Vps4 on ESCRT-III. / Shestakova, Anna; Hanono, Abraham; Drosner, Stacey; Curtiss, Matt; Davies, Brian A.; Katzmann, David J; Babst, Markus.

In: Molecular Biology of the Cell, Vol. 21, No. 6, 15.03.2010, p. 1059-1071.

Research output: Contribution to journalArticle

Shestakova, A, Hanono, A, Drosner, S, Curtiss, M, Davies, BA, Katzmann, DJ & Babst, M 2010, 'Assembly of the AAA ATPase Vps4 on ESCRT-III', Molecular Biology of the Cell, vol. 21, no. 6, pp. 1059-1071. https://doi.org/10.1091/mbc.E09-07-0572
Shestakova A, Hanono A, Drosner S, Curtiss M, Davies BA, Katzmann DJ et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Molecular Biology of the Cell. 2010 Mar 15;21(6):1059-1071. https://doi.org/10.1091/mbc.E09-07-0572
Shestakova, Anna ; Hanono, Abraham ; Drosner, Stacey ; Curtiss, Matt ; Davies, Brian A. ; Katzmann, David J ; Babst, Markus. / Assembly of the AAA ATPase Vps4 on ESCRT-III. In: Molecular Biology of the Cell. 2010 ; Vol. 21, No. 6. pp. 1059-1071.
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