Assays for functionally defined normal and malignant mammary stem cells

Syed Mohammed Musheer Aalam, Philip Anthony Beer, Nagarajan Kannan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The discovery of rare, heterogeneous self-renewing stem cells with shared developmental and molecular features within epithelial components of mammary gland and breast cancers has provided a conceptual framework to understand cellular composition of these tissues and mechanisms that control their number. These normal mammary epithelial stem cells (MaSCs) and breast cancer stem cells (BCSCs) were identified and analyzed using transplant assays (namely mammary repopulating unit (MRU) assay, mammary tumor-initiating cell (TIC) assay), which reveal their latent ability to regenerate respective normal and malignant epithelial tissues with self-renewing units displaying hierarchical cellular differentiation over multiple generations in recipient mice. “Next-generation” methods using “barcoded” normal and malignant mammary cells, with the help of next-generation sequencing (NGS) technology, have revealed hidden complexity and heterogeneous growth potential of MaSCs and BCSCs. Several single markers or combinations of markers have been reported to prospectively enrich MaSCs and BCSCs. Such markers and the extent to which they enrich for MaSCs and BCSCs activity require a critical appraisal. Also, knowledge of the functional assays and their limitations and harmonious reporting of results is a prerequisite to improve our understanding of MaSCs and BCSCs. This chapter describes evolution of the concept of MaSCs and BCSCs, and specific methodologies to investigate them.

Original languageEnglish (US)
Title of host publicationAdvances in Cancer Research
EditorsCurt I. Civin, Tami J. Kingsbury, MinJung Kim, Paul B. Fisher
PublisherAcademic Press Inc.
Pages129-174
Number of pages46
ISBN (Print)9780128149942
DOIs
StatePublished - Jan 1 2019

Publication series

NameAdvances in Cancer Research
Volume141
ISSN (Print)0065-230X
ISSN (Electronic)2162-5557

Fingerprint

Neoplastic Stem Cells
Breast
Stem Cells
Breast Neoplasms
Epithelial Cells
Human Mammary Glands
Epithelium
Technology
Transplants
Growth

Keywords

  • Breast
  • Breast cancer stem cells
  • Lentiviral DNA barcoding
  • Limiting dilution analysis
  • Mammary gland
  • Mammary repopulating unit (MRU) assay
  • Mammary stem cells
  • Tumor initiating cell assay

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Aalam, S. M. M., Beer, P. A., & Kannan, N. (2019). Assays for functionally defined normal and malignant mammary stem cells. In C. I. Civin, T. J. Kingsbury, M. Kim, & P. B. Fisher (Eds.), Advances in Cancer Research (pp. 129-174). (Advances in Cancer Research; Vol. 141). Academic Press Inc.. https://doi.org/10.1016/bs.acr.2018.12.004

Assays for functionally defined normal and malignant mammary stem cells. / Aalam, Syed Mohammed Musheer; Beer, Philip Anthony; Kannan, Nagarajan.

Advances in Cancer Research. ed. / Curt I. Civin; Tami J. Kingsbury; MinJung Kim; Paul B. Fisher. Academic Press Inc., 2019. p. 129-174 (Advances in Cancer Research; Vol. 141).

Research output: Chapter in Book/Report/Conference proceedingChapter

Aalam, SMM, Beer, PA & Kannan, N 2019, Assays for functionally defined normal and malignant mammary stem cells. in CI Civin, TJ Kingsbury, M Kim & PB Fisher (eds), Advances in Cancer Research. Advances in Cancer Research, vol. 141, Academic Press Inc., pp. 129-174. https://doi.org/10.1016/bs.acr.2018.12.004
Aalam SMM, Beer PA, Kannan N. Assays for functionally defined normal and malignant mammary stem cells. In Civin CI, Kingsbury TJ, Kim M, Fisher PB, editors, Advances in Cancer Research. Academic Press Inc. 2019. p. 129-174. (Advances in Cancer Research). https://doi.org/10.1016/bs.acr.2018.12.004
Aalam, Syed Mohammed Musheer ; Beer, Philip Anthony ; Kannan, Nagarajan. / Assays for functionally defined normal and malignant mammary stem cells. Advances in Cancer Research. editor / Curt I. Civin ; Tami J. Kingsbury ; MinJung Kim ; Paul B. Fisher. Academic Press Inc., 2019. pp. 129-174 (Advances in Cancer Research).
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