TY - JOUR
T1 - Ascorbic acid and α-tocopherol as potent modulators on arsenic induced toxicity in mitochondria
AU - Ramanathan, Kadirvel
AU - Shila, Samuel
AU - Kumaran, Sundaram
AU - Panneerselvam, Chinnakkannu
N1 - Funding Information:
This work was supported by grants from Ministry of Environment and Forests, Govt. of India, New Delhi.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Arsenic exists ubiquitously in our environment and various forms of arsenic circulate in air, water, soil and living organisms. Since arsenic compounds have shown to exert their toxicity chiefly by generating reactive oxygen species, we have evaluated the effect of antioxidants ascorbic acid and α-tocopherol on lipid peroxidation, antioxidants and mitochondrial enzymes in liver and kidney of arsenic exposed rats. A significant increase in the level of lipid peroxidation and decrease in the levels of antioxidants and in the activities of mitochondrial enzymes were observed in arsenic intoxicated rats. Co-administration of arsenic treated rats with ascorbic acid and α-tocopherol showed significant reduction in the level of lipid peroxidation and elevation in the levels of ascorbic acid, α-tocopherol, glutathione and total sulfhydryls and in the activities of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, NADH-dehydrogenase and cytochrome c oxidase. From our results, we conclude that ascorbic acid and α-tocopherol alleviate arsenic-induced alterations in mitochondria.
AB - Arsenic exists ubiquitously in our environment and various forms of arsenic circulate in air, water, soil and living organisms. Since arsenic compounds have shown to exert their toxicity chiefly by generating reactive oxygen species, we have evaluated the effect of antioxidants ascorbic acid and α-tocopherol on lipid peroxidation, antioxidants and mitochondrial enzymes in liver and kidney of arsenic exposed rats. A significant increase in the level of lipid peroxidation and decrease in the levels of antioxidants and in the activities of mitochondrial enzymes were observed in arsenic intoxicated rats. Co-administration of arsenic treated rats with ascorbic acid and α-tocopherol showed significant reduction in the level of lipid peroxidation and elevation in the levels of ascorbic acid, α-tocopherol, glutathione and total sulfhydryls and in the activities of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, NADH-dehydrogenase and cytochrome c oxidase. From our results, we conclude that ascorbic acid and α-tocopherol alleviate arsenic-induced alterations in mitochondria.
UR - http://www.scopus.com/inward/record.url?scp=0041706213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041706213&partnerID=8YFLogxK
U2 - 10.1016/S0955-2863(03)00076-7
DO - 10.1016/S0955-2863(03)00076-7
M3 - Article
C2 - 12915223
AN - SCOPUS:0041706213
SN - 0955-2863
VL - 14
SP - 416
EP - 420
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
IS - 7
ER -