TY - JOUR
T1 - Ascorbic acid and α-tocopherol as potent modulators of apoptosis on arsenic induced toxicity in rats
AU - Ramanathan, Kadirvel
AU - Anusuyadevi, Muthuswamy
AU - Shila, Samuel
AU - Panneerselvam, Chinnakannu
PY - 2005/4/10
Y1 - 2005/4/10
N2 - Apoptosis or programmed cell death (PCD) is a genetically regulated cellular, physiological and biochemical suicidal mechanism that plays a crucial role in the development and defense of homeostasis, in which the cell participates in its own demise via a cascade of molecular interactions. PCD can be modulated by various stimuli including infectious agents or drugs. Arsenic is one among inducible toxic agent that triggers apoptosis via free radical generation. Since the generation of free radicals during the metabolism of arsenic is thought to be involved in arsenic toxicosis, understanding the deleterious effects caused by the ROS that attack the vital molecules like DNA has become important. The present work was conducted to evaluate the regulatory effect exerted by Vitamin C and Vitamin E upon the apoptotic process, which can be assessed by the presence of cells with apoptosis associated DNA breaks and characterize the role of TNF-α and caspase-3 in rats intoxicated with arsenic. Male albino rats of wistar strain (120-150 g) were used in this study and are further divided into seven groups. We observed that ascorbate and α-tocopherol selectively altered the extent of DNA damage by reducing TNF-α level and inhibiting the activation of caspase cascade, from these observations it is strongly believed that the present vitamins supplementation perspective, though observed in animal model, will have sustainable curative value among the already afflicted populations, neutralizing impact on freshly emerging arsenicosis scenario and possible proactive protection to those potentially susceptible to arsenicals exposure.
AB - Apoptosis or programmed cell death (PCD) is a genetically regulated cellular, physiological and biochemical suicidal mechanism that plays a crucial role in the development and defense of homeostasis, in which the cell participates in its own demise via a cascade of molecular interactions. PCD can be modulated by various stimuli including infectious agents or drugs. Arsenic is one among inducible toxic agent that triggers apoptosis via free radical generation. Since the generation of free radicals during the metabolism of arsenic is thought to be involved in arsenic toxicosis, understanding the deleterious effects caused by the ROS that attack the vital molecules like DNA has become important. The present work was conducted to evaluate the regulatory effect exerted by Vitamin C and Vitamin E upon the apoptotic process, which can be assessed by the presence of cells with apoptosis associated DNA breaks and characterize the role of TNF-α and caspase-3 in rats intoxicated with arsenic. Male albino rats of wistar strain (120-150 g) were used in this study and are further divided into seven groups. We observed that ascorbate and α-tocopherol selectively altered the extent of DNA damage by reducing TNF-α level and inhibiting the activation of caspase cascade, from these observations it is strongly believed that the present vitamins supplementation perspective, though observed in animal model, will have sustainable curative value among the already afflicted populations, neutralizing impact on freshly emerging arsenicosis scenario and possible proactive protection to those potentially susceptible to arsenicals exposure.
KW - Apoptosis
KW - Arsenic
KW - Caspase-3
KW - DNA fragmentation
KW - TNF-α
KW - Vitamin C
KW - Vitamin E
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UR - http://www.scopus.com/inward/citedby.url?scp=14344256365&partnerID=8YFLogxK
U2 - 10.1016/j.toxlet.2004.12.003
DO - 10.1016/j.toxlet.2004.12.003
M3 - Article
C2 - 15737492
AN - SCOPUS:14344256365
SN - 0378-4274
VL - 156
SP - 297
EP - 306
JO - Toxicology Letters
JF - Toxicology Letters
IS - 2
ER -