Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn, Aswani R. Bolla, Lynn L. Barron, Mercedes E. Gorre, Mansoor S. Mohammed, David W. Bahler, Clemens H.M. Mellink, Marinus H.J. van Oers, Michael J. Keating, Alessandra Ferrajoli, Kevin R. Coombes, Lynne V. Abruzzo, Ryan S. Robetorye

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

Original languageEnglish (US)
Pages (from-to)1276-1281
Number of pages6
JournalLeukemia Research
Volume33
Issue number9
DOIs
StatePublished - Sep 1 2009

Keywords

  • Array CGH
  • Chromosome 22q11
  • Chronic lymphocytic leukemia
  • PRAME gene

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Gunn, S. R., Bolla, A. R., Barron, L. L., Gorre, M. E., Mohammed, M. S., Bahler, D. W., Mellink, C. H. M., van Oers, M. H. J., Keating, M. J., Ferrajoli, A., Coombes, K. R., Abruzzo, L. V., & Robetorye, R. S. (2009). Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene. Leukemia Research, 33(9), 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010