Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn, Aswani R. Bolla, Lynn L. Barron, Mercedes E. Gorre, Mansoor S. Mohammed, David W. Bahler, Clemens H M Mellink, Marinus H J van Oers, Michael J. Keating, Alessandra Ferrajoli, Kevin R. Coombes, Lynne V. Abruzzo, Ryan S. Robetorye

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

Original languageEnglish (US)
Pages (from-to)1276-1281
Number of pages6
JournalLeukemia Research
Volume33
Issue number9
DOIs
StatePublished - Sep 2009
Externally publishedYes

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Chromosome Deletion
B-Cell Chronic Lymphocytic Leukemia
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Messenger RNA
Genes

Keywords

  • Array CGH
  • Chromosome 22q11
  • Chronic lymphocytic leukemia
  • PRAME gene

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene. / Gunn, Shelly R.; Bolla, Aswani R.; Barron, Lynn L.; Gorre, Mercedes E.; Mohammed, Mansoor S.; Bahler, David W.; Mellink, Clemens H M; van Oers, Marinus H J; Keating, Michael J.; Ferrajoli, Alessandra; Coombes, Kevin R.; Abruzzo, Lynne V.; Robetorye, Ryan S.

In: Leukemia Research, Vol. 33, No. 9, 09.2009, p. 1276-1281.

Research output: Contribution to journalArticle

Gunn, SR, Bolla, AR, Barron, LL, Gorre, ME, Mohammed, MS, Bahler, DW, Mellink, CHM, van Oers, MHJ, Keating, MJ, Ferrajoli, A, Coombes, KR, Abruzzo, LV & Robetorye, RS 2009, 'Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene', Leukemia Research, vol. 33, no. 9, pp. 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010
Gunn, Shelly R. ; Bolla, Aswani R. ; Barron, Lynn L. ; Gorre, Mercedes E. ; Mohammed, Mansoor S. ; Bahler, David W. ; Mellink, Clemens H M ; van Oers, Marinus H J ; Keating, Michael J. ; Ferrajoli, Alessandra ; Coombes, Kevin R. ; Abruzzo, Lynne V. ; Robetorye, Ryan S. / Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene. In: Leukemia Research. 2009 ; Vol. 33, No. 9. pp. 1276-1281.
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abstract = "We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15{\%}), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.",
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