Aptamer-based electrochemical biosensor for interferon gamma detection

Ying Liu, Nazgul Tuleouva, Erlan Ramanculov, Alexander Revzin

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.

Original languageEnglish (US)
Pages (from-to)8131-8136
Number of pages6
JournalAnalytical Chemistry
Volume82
Issue number19
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

Fingerprint

Biosensors
Interferons
Interferon-gamma
Methylene Blue
Nucleotide Aptamers
Immunology
Electrodes
Voltammetry
Washing
Gold
Self assembly
Urea
Blood Proteins
Buffers
Molecules
Electrons
Monitoring
DNA
Oxidation-Reduction

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Aptamer-based electrochemical biosensor for interferon gamma detection. / Liu, Ying; Tuleouva, Nazgul; Ramanculov, Erlan; Revzin, Alexander.

In: Analytical Chemistry, Vol. 82, No. 19, 01.10.2010, p. 8131-8136.

Research output: Contribution to journalArticle

Liu, Ying ; Tuleouva, Nazgul ; Ramanculov, Erlan ; Revzin, Alexander. / Aptamer-based electrochemical biosensor for interferon gamma detection. In: Analytical Chemistry. 2010 ; Vol. 82, No. 19. pp. 8131-8136.
@article{6e853ec6ede149b881e14429a198046f,
title = "Aptamer-based electrochemical biosensor for interferon gamma detection",
abstract = "In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.",
author = "Ying Liu and Nazgul Tuleouva and Erlan Ramanculov and Alexander Revzin",
year = "2010",
month = "10",
day = "1",
doi = "10.1021/ac101409t",
language = "English (US)",
volume = "82",
pages = "8131--8136",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "19",

}

TY - JOUR

T1 - Aptamer-based electrochemical biosensor for interferon gamma detection

AU - Liu, Ying

AU - Tuleouva, Nazgul

AU - Ramanculov, Erlan

AU - Revzin, Alexander

PY - 2010/10/1

Y1 - 2010/10/1

N2 - In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.

AB - In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.

UR - http://www.scopus.com/inward/record.url?scp=77957304421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957304421&partnerID=8YFLogxK

U2 - 10.1021/ac101409t

DO - 10.1021/ac101409t

M3 - Article

C2 - 20815336

AN - SCOPUS:77957304421

VL - 82

SP - 8131

EP - 8136

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 19

ER -