Application of structural rigidity analysis to assess fidelity of healed fractures in rat femurs with critical defects

Ara Nazarian, Lina Pezzella, Alan Tseng, Stephen Baldassarri, David Zurakowski, Christopher H. Evans, Brian D. Snyder

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Approximately 6 million fractures occur each year in the United States, with an estimated medical and loss of productivity cost of $99 billion. As our population ages, it can only be expected that these numbers will continue to rise. While there have been recent advances in available treatments for fractures, assessment of the healing process remains a subjective process. This study aims to demonstrate the use of micro-computed tomography (μCT)based structural rigidity analysis to accurately and quantitatively assess the progression of fracture healing over time in a rat model. The femora of rats with simulated lytic defects were injected with human BMP-2 cDNA at various time points postinjury (t = 0, 1, 5, 10 days) to accelerate fracture healing, harvested 56 days from time of injury, and subjected to μCT imaging to obtain cross-sectional data that were used to compute torsional rigidity. The specimens then underwent torsional testing to failure using a previously described pure torsional testing system. Strong correlations were found between measured torsional rigidity and computed torsional rigidity as calculated from both average (R2 = 0.63) and minimum (R2 = 0.81) structural rigidity data. While both methods were well correlated across the entire data range, minimum torsional rigidity was a better descriptor of bone strength, as seen by a higher Pearson coefficient and smaller y-intercept. These findings suggest considerable promise in the use of structural rigidity analysis of μCT data to accurately and quantitatively measure fracture-healing progression.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalCalcified Tissue International
Volume86
Issue number5
DOIs
StatePublished - May 2010

Keywords

  • Fracture healing
  • Healing strength
  • Rat model
  • Segmental defect
  • Structural rigidity analysis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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