Appearance of neurons in the developing chick gut

C. L. Fairman, M. Clagett-Dame, Vanda A Lennon, M. L. Epstein

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The enteric nervous system is i formed from neural crest-derived cells. These cells enter the gut, migrate, proliferate, and ultimately differentiate into neurons and glia. We have used a human anti-neuronal autoantibody (ANNA- l), which recognizes neuron-specific -binding proteins of the Hu family as an early marker of neuronal phenotype, to study the appearance of enteric neurons in the developing chicken gut. Immunoreactive cells appear first in the gizzard primordium at E3.5 and are found at progressively more caudal locations in the gut as development proceeds. Nascent neurons are present at the yolk stalk at E4.5, at the ileocecal junction at E6.5, and within the rectum at E7.5-8.5. Neurons appear slightly later in the esophagus. Aggregates of cells resembling developing ganglia were first observed at E6.5 in the distal esophagus and at E8.5 in the proximal esophagus. A small number of cells appeared in the vagus nerve trunks at E4.5 and that number increased at E7.5-8.5. Immunoreactive cells were also found in the sympatho-aortic plexus between the mesonephri and in the dorsal mesentery. These cells appeared to coalesce and form the ganglionated Nerve of Remak which contained positive cells at E3.5. This Nerve extended to the yolk stalk at E4.5 and to duodenum at E6.5. We conclude that the appearance of nascent neurons occurs first in the gizzard and proceeds more rapidly in a distal than proximal direction along the gut. Furthermore, cells that appear to be nascent neurons are found in the vagus and in the dorsal mesentery.

Original languageEnglish (US)
Pages (from-to)192-201
Number of pages10
JournalDevelopmental Dynamics
Volume204
Issue number2
StatePublished - 1995

Fingerprint

Neurons
Esophagus
Avian Gizzard
Mesentery
Enteric Nervous System
Vagus Nerve
Neural Crest
Duodenum
Rectum
Neuroglia
Ganglia
Autoantibodies
Chickens
Carrier Proteins
Cell Count
Phenotype

Keywords

  • ANNA-1
  • Anti-neuronal nuclear antibodies
  • Chick embryo
  • Enteric neuron
  • Hu
  • Nerve of Remak
  • Neuron development
  • RNA binding proteins
  • Vagus nerve

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Fairman, C. L., Clagett-Dame, M., Lennon, V. A., & Epstein, M. L. (1995). Appearance of neurons in the developing chick gut. Developmental Dynamics, 204(2), 192-201.

Appearance of neurons in the developing chick gut. / Fairman, C. L.; Clagett-Dame, M.; Lennon, Vanda A; Epstein, M. L.

In: Developmental Dynamics, Vol. 204, No. 2, 1995, p. 192-201.

Research output: Contribution to journalArticle

Fairman, CL, Clagett-Dame, M, Lennon, VA & Epstein, ML 1995, 'Appearance of neurons in the developing chick gut', Developmental Dynamics, vol. 204, no. 2, pp. 192-201.
Fairman CL, Clagett-Dame M, Lennon VA, Epstein ML. Appearance of neurons in the developing chick gut. Developmental Dynamics. 1995;204(2):192-201.
Fairman, C. L. ; Clagett-Dame, M. ; Lennon, Vanda A ; Epstein, M. L. / Appearance of neurons in the developing chick gut. In: Developmental Dynamics. 1995 ; Vol. 204, No. 2. pp. 192-201.
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