Apparent cleavage of poly(ADP-ribose) polymerase in non-apoptotic mouse LTA cells: An artifact of cross-reactive secondary antibody

I. Imawati Budihardjo, Guy G. Poirier, Scott H Kaufmann

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Proteolytic cleavage of the nuclear enzyme poly(ADP-ribose) polymerase (PARP) to fragments of 89 kD and 24 kD is widely observed during apoptotic cell death. In the present study, labelling of a M(r) ~ 89000 polypeptide was demonstrated in untreated mouse LTA cells during probing of immunoblots with C-2-10 monoclonal anti-PARP antibody. The source of the labeling was traced to the secondary antibody preparation, which labeled a M(r) ~ 89000 polypeptide in murine LTA cells but not in human cells. These observations indicate that assessment of PARP cleavage must be (1) performed with appropriate controls when new cell lines are investigated and (2) carefully interpreted in light of additional biochemical or morphological data demonstrating apoptotic changes.

Original languageEnglish (US)
Pages (from-to)245-249
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume178
Issue number1-2
DOIs
StatePublished - 1998

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Poly(ADP-ribose) Polymerases
Artifacts
Labeling
Antibodies
Cells
Peptides
Cell death
Cell Death
Cell Line
Enzymes

Keywords

  • Antibody cross-reactivity
  • Apoptosis
  • Poly(ADP-ribose) polymerase

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Apparent cleavage of poly(ADP-ribose) polymerase in non-apoptotic mouse LTA cells : An artifact of cross-reactive secondary antibody. / Budihardjo, I. Imawati; Poirier, Guy G.; Kaufmann, Scott H.

In: Molecular and Cellular Biochemistry, Vol. 178, No. 1-2, 1998, p. 245-249.

Research output: Contribution to journalArticle

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