Apocynin attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung model

Chih Feng Chian, Chi Huei Chiang, Chu Yuan-Jung, Chiao Hui Chuang, Shiou Ling Liu, Jheng Yi-Han, Haibo Zhang, Jay H. Ryu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Apocynin (Apo) suppresses the generation of reactive oxygen species that are implicated in lipopolysaccharide (LPS)-induced lung injury (LPSLI). We thus hypothesized that Apo may attenuate LPSLI. In addition, we explored the cellular and molecular mechanisms of Apo treatment in LPSLI. Lipopolysaccharide-induced lung injury was induced by intratracheal instillation of 10 mg/kg LPS in isolated and perfused rat lung model. Apocynin was administered in the perfusate at 15 min before LPS was administered. Hemodynamics, lung injury indices, inflammatory responses, and activation of apoptotic pathways were assessed. There was an increase in lung vascular permeability associated with lung weight gain after LPS exposure. The levels of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), macrophage inflammatory protein 2, H 2O 2, and albumin increased in the bronchoalveolar lavage fluid. Adhesion molecule of neutrophil (CD31) was upregulated. The expression of TNF-α, IL-1β, glutathione, myeloperoxidase, JNK, P38, caspase 3, p-AKT, and plasminogen activator inhibitor 1 in lung tissue was greater in the LPS groups when compared with the control group. Upregulation and activation of nuclear factor κB occurred along with increased histopathologic lung injury score in LPSLI. The Apo attenuated these inflammatory responses including the levels of CD31, H 2O 2, TNF-α, IL-1β, myeloperoxidase, P38, and nuclear factor κB along with downregulation of apoptosis as reflected by caspase 3 and p-AKT. In addition, Apo attenuated the increase in lung weight, bronchoalveolar lavage fluid albumin content, and the histopathologic lung injury score. In conclusion, LPSLI is associated with increased inflammatory responses, apoptosis, and coagulation. The administration of Apo attenuates LPSLI through downregulation of the inflammatory responses and apoptosis.

Original languageEnglish (US)
Pages (from-to)196-202
Number of pages7
JournalShock
Volume38
Issue number2
DOIs
StatePublished - Aug 1 2012

Keywords

  • ARDS
  • Apocynin
  • LPS
  • MAPK
  • NADPH
  • NF-κB
  • inflammation

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Fingerprint Dive into the research topics of 'Apocynin attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung model'. Together they form a unique fingerprint.

  • Cite this

    Chian, C. F., Chiang, C. H., Yuan-Jung, C., Chuang, C. H., Liu, S. L., Yi-Han, J., Zhang, H., & Ryu, J. H. (2012). Apocynin attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung model. Shock, 38(2), 196-202. https://doi.org/10.1097/SHK.0b013e31825a1982