Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions

Liang Wang, William Paradee, Chadwick Mullins, Ravi Shridhar, Rita Rosati, Charles M. Wilke, Thomas W. Glover, David I Smith

Research output: Contribution to journalArticle

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Abstract

The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin induction of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with breakpoints in the 3p14.2 region. These breakpoints were localized to two distinct clusters, separated by 200 kb, that lie on either side of a region of frequent breakage within FRA3B as observed by FISH analysis. Seven proximal aphidicolin-induced breakpoints were localized at or near the end of a THE element. The THE-1 element is flanked by LINE and Alu repetitive elements. The eight distal aphidicolin- induced breakpoints clustered in a region capable of forming multiple hairpin-like structures. Thus repetitive elements and hairpin-like structures may be responsible for chromosome fragility in this region.

Original languageEnglish (US)
Pages (from-to)485-488
Number of pages4
JournalGenomics
Volume41
Issue number3
DOIs
StatePublished - May 1 1997

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Aphidicolin
Chromosome Fragility
Alu Elements
Chromosomes, Human, Pair 3
Hybrid Cells
Human Genome
Chromosomes
Kidney
Lung
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Wang, L., Paradee, W., Mullins, C., Shridhar, R., Rosati, R., Wilke, C. M., ... Smith, D. I. (1997). Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions. Genomics, 41(3), 485-488. https://doi.org/10.1006/geno.1997.4690

Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions. / Wang, Liang; Paradee, William; Mullins, Chadwick; Shridhar, Ravi; Rosati, Rita; Wilke, Charles M.; Glover, Thomas W.; Smith, David I.

In: Genomics, Vol. 41, No. 3, 01.05.1997, p. 485-488.

Research output: Contribution to journalArticle

Wang, L, Paradee, W, Mullins, C, Shridhar, R, Rosati, R, Wilke, CM, Glover, TW & Smith, DI 1997, 'Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions', Genomics, vol. 41, no. 3, pp. 485-488. https://doi.org/10.1006/geno.1997.4690
Wang L, Paradee W, Mullins C, Shridhar R, Rosati R, Wilke CM et al. Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions. Genomics. 1997 May 1;41(3):485-488. https://doi.org/10.1006/geno.1997.4690
Wang, Liang ; Paradee, William ; Mullins, Chadwick ; Shridhar, Ravi ; Rosati, Rita ; Wilke, Charles M. ; Glover, Thomas W. ; Smith, David I. / Aphidicolin-induced FRA3B breakpoints cluster in two distinct regions. In: Genomics. 1997 ; Vol. 41, No. 3. pp. 485-488.
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