Antiviral effects of a transgenic RNA-dependent RNA polymerase

Jason Kerkvliet, Louisa Papke, Moses Rodriguez

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Transgenic expression of the RNA-dependent RNA polymerase 3Dpol inhibited infection of Theiler's murine encephalitis virus (TMEV), a picornavirus from which it was derived. Here, we infected 3Dpol transgenic mice with another picornavirus, as well as an alphaherpesvirus and a rhabdovirus. 3Dpol transgenic FVB mice had significantly lower viral loads and survived longer after infection with all three types of viruses than nontransgenic FVB mice. Viral inhibition among three different types of virus by transgenic 3Dpol suggests that the mechanism of action is not the direct interference with picornaviral 3Dpol but instead may be the changing of host cells to an antiviral state before or after viral infection occurs, as basal interferon levels were higher in 3Dpol transgenic mice before infection. Further study of this mechanism may open new possibilities for future antiviral therapy.

Original languageEnglish (US)
Pages (from-to)621-625
Number of pages5
JournalJournal of virology
Volume85
Issue number1
DOIs
StatePublished - Jan 1 2011

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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