Antioxidant vitamins induce angiogenesis in the normal pig kidney

Elena Daghini, Xiang Yang Zhu, Daniele Versari, Michael D. Bentley, Claudio Napoli, Amir Lerman, Lilach O Lerman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effects of chronic supplementation with antioxidant vitamins on angiogenesis are controversial. The aim of the present study was to evaluate in kidneys of normal pigs the effect of chronic supplementation with vitamins E and C, at doses that are effective in reducing oxidative stress and attenuating angiogenesis under pathological conditions. Domestic pigs were randomized to receive a 12-wk normal diet without (n = 6) or with antioxidant vitamins supplementation (1g/day vitamin C, 100 IU·kg-1·day -1 vitamin E; n = 6). Electron beam computed tomography (CT) was used to evaluate renal cortical vascular function in vivo, and micro-CT was to assess the spatial density and average diameter of cortical microvessels (diameter <500 μm) ex vivo. Oxidative stress and expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF)-1α were evaluated in renal tissue. The effects of increasing concentrations of the same vitamins on redox status and angiogenesis were also evaluated in human umbilical vascular endothelial cells (HUVEC). Compared with normal pigs, the density of cortical transmural microvessels was significantly greater in vitamin-supplemented pigs (149.0 -± 11.7 vs. 333.8 ±- 48.1 vessel/cm2, P < 0.05), whereas the cortical perfusion response to ACh was impaired. This was accompanied by a significant increase in tissue oxidative stress and levels of VEGF and HIF-1α. A low dose of antioxidant decreased, whereas a high dose increased, HUVEC oxidative stress and angiogenesis, which was partly mediated by hydrogen peroxide. Antioxidant vitamin supplementation can increase tissue oxidative redox and microvascular proliferation in the normal kidney, probably due to a biphasic effect that depends on basal redox balance.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number1
DOIs
StatePublished - Jul 2007

Fingerprint

Vitamins
Swine
Antioxidants
Kidney
Oxidative Stress
Oxidation-Reduction
Umbilicus
Hypoxia-Inducible Factor 1
Microvessels
Vitamin E
Vascular Endothelial Growth Factor A
Ascorbic Acid
Endothelial Cells
Pathologic Neovascularization
Sus scrofa
X Ray Computed Tomography
Hydrogen Peroxide
Blood Vessels
Perfusion
Tomography

Keywords

  • Oxidative stress

ASJC Scopus subject areas

  • Physiology

Cite this

Antioxidant vitamins induce angiogenesis in the normal pig kidney. / Daghini, Elena; Zhu, Xiang Yang; Versari, Daniele; Bentley, Michael D.; Napoli, Claudio; Lerman, Amir; Lerman, Lilach O.

In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 1, 07.2007.

Research output: Contribution to journalArticle

Daghini, Elena ; Zhu, Xiang Yang ; Versari, Daniele ; Bentley, Michael D. ; Napoli, Claudio ; Lerman, Amir ; Lerman, Lilach O. / Antioxidant vitamins induce angiogenesis in the normal pig kidney. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 293, No. 1.
@article{08110bb3f6af4bbfb188900ba25fcad7,
title = "Antioxidant vitamins induce angiogenesis in the normal pig kidney",
abstract = "The effects of chronic supplementation with antioxidant vitamins on angiogenesis are controversial. The aim of the present study was to evaluate in kidneys of normal pigs the effect of chronic supplementation with vitamins E and C, at doses that are effective in reducing oxidative stress and attenuating angiogenesis under pathological conditions. Domestic pigs were randomized to receive a 12-wk normal diet without (n = 6) or with antioxidant vitamins supplementation (1g/day vitamin C, 100 IU·kg-1·day -1 vitamin E; n = 6). Electron beam computed tomography (CT) was used to evaluate renal cortical vascular function in vivo, and micro-CT was to assess the spatial density and average diameter of cortical microvessels (diameter <500 μm) ex vivo. Oxidative stress and expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF)-1α were evaluated in renal tissue. The effects of increasing concentrations of the same vitamins on redox status and angiogenesis were also evaluated in human umbilical vascular endothelial cells (HUVEC). Compared with normal pigs, the density of cortical transmural microvessels was significantly greater in vitamin-supplemented pigs (149.0 -± 11.7 vs. 333.8 ±- 48.1 vessel/cm2, P < 0.05), whereas the cortical perfusion response to ACh was impaired. This was accompanied by a significant increase in tissue oxidative stress and levels of VEGF and HIF-1α. A low dose of antioxidant decreased, whereas a high dose increased, HUVEC oxidative stress and angiogenesis, which was partly mediated by hydrogen peroxide. Antioxidant vitamin supplementation can increase tissue oxidative redox and microvascular proliferation in the normal kidney, probably due to a biphasic effect that depends on basal redox balance.",
keywords = "Oxidative stress",
author = "Elena Daghini and Zhu, {Xiang Yang} and Daniele Versari and Bentley, {Michael D.} and Claudio Napoli and Amir Lerman and Lerman, {Lilach O}",
year = "2007",
month = "7",
doi = "10.1152/ajprenal.00475.2006",
language = "English (US)",
volume = "293",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Antioxidant vitamins induce angiogenesis in the normal pig kidney

AU - Daghini, Elena

AU - Zhu, Xiang Yang

AU - Versari, Daniele

AU - Bentley, Michael D.

AU - Napoli, Claudio

AU - Lerman, Amir

AU - Lerman, Lilach O

PY - 2007/7

Y1 - 2007/7

N2 - The effects of chronic supplementation with antioxidant vitamins on angiogenesis are controversial. The aim of the present study was to evaluate in kidneys of normal pigs the effect of chronic supplementation with vitamins E and C, at doses that are effective in reducing oxidative stress and attenuating angiogenesis under pathological conditions. Domestic pigs were randomized to receive a 12-wk normal diet without (n = 6) or with antioxidant vitamins supplementation (1g/day vitamin C, 100 IU·kg-1·day -1 vitamin E; n = 6). Electron beam computed tomography (CT) was used to evaluate renal cortical vascular function in vivo, and micro-CT was to assess the spatial density and average diameter of cortical microvessels (diameter <500 μm) ex vivo. Oxidative stress and expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF)-1α were evaluated in renal tissue. The effects of increasing concentrations of the same vitamins on redox status and angiogenesis were also evaluated in human umbilical vascular endothelial cells (HUVEC). Compared with normal pigs, the density of cortical transmural microvessels was significantly greater in vitamin-supplemented pigs (149.0 -± 11.7 vs. 333.8 ±- 48.1 vessel/cm2, P < 0.05), whereas the cortical perfusion response to ACh was impaired. This was accompanied by a significant increase in tissue oxidative stress and levels of VEGF and HIF-1α. A low dose of antioxidant decreased, whereas a high dose increased, HUVEC oxidative stress and angiogenesis, which was partly mediated by hydrogen peroxide. Antioxidant vitamin supplementation can increase tissue oxidative redox and microvascular proliferation in the normal kidney, probably due to a biphasic effect that depends on basal redox balance.

AB - The effects of chronic supplementation with antioxidant vitamins on angiogenesis are controversial. The aim of the present study was to evaluate in kidneys of normal pigs the effect of chronic supplementation with vitamins E and C, at doses that are effective in reducing oxidative stress and attenuating angiogenesis under pathological conditions. Domestic pigs were randomized to receive a 12-wk normal diet without (n = 6) or with antioxidant vitamins supplementation (1g/day vitamin C, 100 IU·kg-1·day -1 vitamin E; n = 6). Electron beam computed tomography (CT) was used to evaluate renal cortical vascular function in vivo, and micro-CT was to assess the spatial density and average diameter of cortical microvessels (diameter <500 μm) ex vivo. Oxidative stress and expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF)-1α were evaluated in renal tissue. The effects of increasing concentrations of the same vitamins on redox status and angiogenesis were also evaluated in human umbilical vascular endothelial cells (HUVEC). Compared with normal pigs, the density of cortical transmural microvessels was significantly greater in vitamin-supplemented pigs (149.0 -± 11.7 vs. 333.8 ±- 48.1 vessel/cm2, P < 0.05), whereas the cortical perfusion response to ACh was impaired. This was accompanied by a significant increase in tissue oxidative stress and levels of VEGF and HIF-1α. A low dose of antioxidant decreased, whereas a high dose increased, HUVEC oxidative stress and angiogenesis, which was partly mediated by hydrogen peroxide. Antioxidant vitamin supplementation can increase tissue oxidative redox and microvascular proliferation in the normal kidney, probably due to a biphasic effect that depends on basal redox balance.

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=34548042311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548042311&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00475.2006

DO - 10.1152/ajprenal.00475.2006

M3 - Article

C2 - 17429028

AN - SCOPUS:34548042311

VL - 293

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1

ER -