Abstract
To identify whether sera containing antinuclear antibody (ANA) from patients with juvenile rheumatoid arthritis (JRA) recognize a common antigen, extracts of HeLa nuclei were prepared by incubating the nuclei with buffers of sequentially increasing salt concentrations (0.1 M, 0.3 M, and 0.5 M NaCl). Western blot analysis using sera from 82 patients with JRA revealed that 63% of ANA positive JRA sera reacted with 2 bands (50-40 kDa) in the 0.3 M extract whereas only 14% of ANA negative JRA sera had antibody to these bands. Antibody to the 50-40 kDa antigen(s) strongly correlated with the presence of ANA by immunofluorescence and was found in 70% of patients with iritis; however, it did not correlate with disease subgroup or disease activity.
Original language | English (US) |
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Pages (from-to) | 949-954 |
Number of pages | 6 |
Journal | Journal of Rheumatology |
Volume | 16 |
Issue number | 7 |
State | Published - 1989 |
ASJC Scopus subject areas
- Rheumatology
- Immunology and Allergy
- Immunology