Antigenic requirements for induction of hyperacute EAE

Vanda A Lennon, F. C. Westall, M. Thompson

Research output: Contribution to journalArticle

Abstract

In rats a hyperacute variant of experimental autoimmune encephalomyelitis (HEAE), distinguisable clinically and histologically from ordinary EAE, is described using whole nervous tissue as immunogen and B. pertussis as adjuvant. Since EAE and HEAE overlap histologically and immunologically, they provide the first experimental evidence distinguishable supporting the concept that certain human demyelinating diseases represent a spectrum of autoallergy. On repeated testing of purified myelin basic proteins (BP's) of six mammalian species (guinea pig, rat, human, ox, rabbit and pig), only guinea pig BP, in combination with Freund's complete adjuvant and B. pertussis, was found capable of inducing HEAE. The mid peptide (residues 45 to 89) was identified as the region of the guinea pig BP molecule inducing HEAE. Comparison of amino acid sequences of this region of BP's from various species allowed further definition of the immunogenic determinant(s) unique to guinea pig BP responsible for HEAE induction. The data suggest that an immunogenic determinant unique to the guinea pig mid peptide is either acting as a secondary encephalitogenic determinant or alternatively as a non encephalitogenic carrier preferentially inducing helper T cells to recruit IgE secreting B cells in response to the encephalitogenic determinant.

Original languageEnglish (US)
Pages (from-to)4137
Number of pages1
JournalFederation Proceedings
Volume34
Issue number3
StatePublished - 1975
Externally publishedYes

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Autoimmune Experimental Encephalomyelitis
Guinea Pigs
Whooping Cough
Proteins
Nerve Tissue
Peptides
Myelin Basic Protein
Freund's Adjuvant
Demyelinating Diseases
Helper-Inducer T-Lymphocytes
Immunoglobulin E
Amino Acid Sequence
B-Lymphocytes
Swine
Rabbits

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Antigenic requirements for induction of hyperacute EAE. / Lennon, Vanda A; Westall, F. C.; Thompson, M.

In: Federation Proceedings, Vol. 34, No. 3, 1975, p. 4137.

Research output: Contribution to journalArticle

Lennon, VA, Westall, FC & Thompson, M 1975, 'Antigenic requirements for induction of hyperacute EAE', Federation Proceedings, vol. 34, no. 3, pp. 4137.
Lennon, Vanda A ; Westall, F. C. ; Thompson, M. / Antigenic requirements for induction of hyperacute EAE. In: Federation Proceedings. 1975 ; Vol. 34, No. 3. pp. 4137.
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