Abstract
The presence of IL-12 during antigen stimulation instructs naive CD8+ T cells for long-term effector responses, but their mechanisms of collaboration are not understood completely. Herein, we report that CD8+ T cells (OT-I T cells) stimulated with antigen for a longer duration show enhanced sensitization to IL-12 as a result of Erk1/2-dependent, increased Ets-1 phosphorylation and subsequent increases in IL-12Rβ2 expression. Correspondingly, naive OT-I T cells stimulated by antigen for a longer duration in the presence of IL-12, irrespective of frequency of APCs, show robust effector maturation and mount long-term antigen-recall responses upon adoptive transfer. These results identify the role of antigen strength-dependent Erk1/2 activation for Ets-1-mediated collaboration with IL-12 in CD8+ T cells. J. Leukoc. Biol. 87: 257-263; 2010.
Original language | English (US) |
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Pages (from-to) | 257-263 |
Number of pages | 7 |
Journal | Journal of Leukocyte Biology |
Volume | 87 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2010 |
Keywords
- CTL
- Ifn-γ
- ·
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology