Antigen-induced Erk1/2 activation regulates Ets-1-mediated sensitization of CD8+ T cells for IL-12 responses

Qingsheng Li, Cheryl Eppolito, Kunle Odunsi, Protul A. Shrikant

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The presence of IL-12 during antigen stimulation instructs naive CD8+ T cells for long-term effector responses, but their mechanisms of collaboration are not understood completely. Herein, we report that CD8+ T cells (OT-I T cells) stimulated with antigen for a longer duration show enhanced sensitization to IL-12 as a result of Erk1/2-dependent, increased Ets-1 phosphorylation and subsequent increases in IL-12Rβ2 expression. Correspondingly, naive OT-I T cells stimulated by antigen for a longer duration in the presence of IL-12, irrespective of frequency of APCs, show robust effector maturation and mount long-term antigen-recall responses upon adoptive transfer. These results identify the role of antigen strength-dependent Erk1/2 activation for Ets-1-mediated collaboration with IL-12 in CD8+ T cells. J. Leukoc. Biol. 87: 257-263; 2010.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalJournal of Leukocyte Biology
Volume87
Issue number2
DOIs
StatePublished - Feb 1 2010

Keywords

  • CTL
  • Ifn-γ
  • ·

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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