Antibody responses to Simian Virus 40 T antigen: A case-control study of non-Hodgkin lymphoma

Eric A. Engels, Jinbo Chen, Patricia Hartge, James R Cerhan, Scott Davis, Richard K. Severson, Wendy Cozen, Raphael P. Viscidi

Research output: Contribution to journalArticle

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Abstract

A possible role for SV40, a macaque polyomavirus, in non-Hodgkin lymphoma (NHL) in humans was raised recently by the reported detection of SV40 DNA in tumor tissue. Animals with SV40-induced tumors frequently produce high-level antibodies against T antigen, the SV40 oncoprotein. In this study, we assessed whether SV40 T antibody measured in humans supported a relationship between SV40 and NHL. Subjects were sampled from a U.S. population-based case-control study of NHL, according to presence of antibodies against capsids of SV40 and BK, a related human polyomavirus (n = 85 cases, n = 95 controls). T antibody was measured by enzyme immunoassay. We also evaluated serum specimens from SV40-infected and SV40-uninfected macaques (n = 19 and n = 8, respectively), SV40-uninfected hamsters (n = 5), and hamsters with SV40-induced tumors (n = 10). Hamsters with SV40-induced tumors all produced robust SV40 T antibody (median absorbance, 0.99), whereas SV40-uninfected hamsters and macaques had much lower levels (median absorbance, 0.05 and 0.04, respectively). NHL cases, controls, and SV40-infected macaques resembled these latter two groups, generally showing only low-level T antibody (median absorbance, 0.03, 0.04, and 0.04, respectively). Overall, only five cases (6%) and five controls (5%) had T antibody responses classified as seropositive (odds ratio, 1.2; 95% confidence interval, 0.3-4.6). Interestingly, all 10 humans with T antibody responses also showed antibody responses to BK capsid. We found no association between the presence of T antibody and NHL, arguing against SV40 as a cause of NHL. Infrequent and low-level T antibody responses among humans could represent cross-reactivity to BK virus T antigen.

Original languageEnglish (US)
Pages (from-to)521-524
Number of pages4
JournalCancer Epidemiology Biomarkers and Prevention
Volume14
Issue number2
DOIs
StatePublished - Feb 2005

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Simian virus 40
Viral Tumor Antigens
Non-Hodgkin's Lymphoma
Antibody Formation
Case-Control Studies
Macaca
Antibodies
Cricetinae
Polyomavirus
Capsid
Neoplasms
BK Virus
Polyomavirus Transforming Antigens
Oncogene Proteins
Immunoenzyme Techniques
Odds Ratio
Confidence Intervals
DNA
Serum
Population

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Antibody responses to Simian Virus 40 T antigen : A case-control study of non-Hodgkin lymphoma. / Engels, Eric A.; Chen, Jinbo; Hartge, Patricia; Cerhan, James R; Davis, Scott; Severson, Richard K.; Cozen, Wendy; Viscidi, Raphael P.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 14, No. 2, 02.2005, p. 521-524.

Research output: Contribution to journalArticle

Engels, Eric A. ; Chen, Jinbo ; Hartge, Patricia ; Cerhan, James R ; Davis, Scott ; Severson, Richard K. ; Cozen, Wendy ; Viscidi, Raphael P. / Antibody responses to Simian Virus 40 T antigen : A case-control study of non-Hodgkin lymphoma. In: Cancer Epidemiology Biomarkers and Prevention. 2005 ; Vol. 14, No. 2. pp. 521-524.
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