Antibody-mediated neutrophil depletion preserves pulmonary vasomotor function

Brett C. Sheridan, Robert C. McIntyre, Daniel R. Meldrum, Joseph C. Cleveland, Jeanette Agrafojo, John H. Eisenach, Alden H. Harken, David A. Fullerton

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7 Scopus citations

Abstract

Neutrophil depletion is commonly used to examine the role of neutrophils in lung injury. However, the effect of neutrophil depletion per se on mechanisms of pulmonary vascular smooth muscle relaxation is unknown. The purpose of this study was to examine the effect of neutropenia on the following mechanisms of cGMP-mediated pulmonary vasorelaxation: (1) receptor- dependent endothelium-dependent relaxation (response to acetylcholine (ACh)), (2) receptor-independent endothelium-dependent relaxation (response to the calcium ionophore A23187), and (3) endothelium-independent relaxation (response to sodium nitroprusside (SNP)). Neutropenia (<75 neutrophils/μl) was induced with anti-neutrophil antibody serum 24 hr prior to lung harvest in five rats. Saline-injected rats were controls (n = 5). Dose-response curves to ACh, A23187, and SNP were generated in isolated pulmonary artery rings preconstricted with phenylepherine. Statistical comparison was performed using one-way ANOVA with post-hoc Bonferroni-Dunn, and P < 0.05 was accepted as significant. Relaxation to ACh, A23187, and SNP was complete in both control and neutropenic rats. Thus, antibody-mediated depletion does not impair endothelial-dependent or -independent cGMP-mediated pulmonary vasorelaxation.

Original languageEnglish (US)
Pages (from-to)74-78
Number of pages5
JournalJournal of Surgical Research
Volume62
Issue number1
DOIs
StatePublished - Apr 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Surgery

Cite this

Sheridan, B. C., McIntyre, R. C., Meldrum, D. R., Cleveland, J. C., Agrafojo, J., Eisenach, J. H., Harken, A. H., & Fullerton, D. A. (1996). Antibody-mediated neutrophil depletion preserves pulmonary vasomotor function. Journal of Surgical Research, 62(1), 74-78. https://doi.org/10.1006/jsre.1996.0176