Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals

Lucy Pemberton, Joyce Taylor-Papadimitriou, Sandra J. Gendler

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

An antiserum against the carboxy-terminal seventeen amino acids of the human MUC1 mucin has been raised and extensively characterized. This antiserum, CT1, immunoprecipitates two high molecular weight polymorphic bands (>200 kDa) from a metabolically labelled breast cancer cell line corresponding to the two alleles which have previously been shown to contain different numbers of a twenty amino acid repeat. The CT1 antiserum reacted with tissues from many mammalian species and immunoprecipitated large polymorphic proteins, suggesting that the cytoplasmic portion of the molecule is well conserved. The cell and tissue distribution of Muc-1 mucin in the mouse has been studied by immunocytochemistry. This protein is abundant at the apical surfaces of epithelial tissues and is found expressed in the stomach, kidney, mammary gland, pancreas, salivary gland, lung, trachea, uterus, cervix and vagina.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number1
DOIs
StatePublished - May 29 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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