Oxidized forms of LDL (oxLDL) are known to be atherogenic and immunogenic. Antibodies against oxLDL (anti-oxLDL) have also been associated with acceleration of atherosclerosis through their ability to increase oxLDL uptake. The Louisiana Coushatta Indians are known to have a rate of cardiovascular death that is 5× higher than the rest of the state. Therefore, in order to delineate the factors underlying their cardiovascular disease, we have begun to investigate the presence of anti-oxLDL in tribal members. Seurm IgG antibody levels against native LDL (nLDL) and oxLDL were determined via ELISA. LDL was extracted from human serum, and oxLDL was prepared by dialysis against CuSO4 Data were expressed as the ratio of the O.D. of anti-oxLDL to the O.D. of anti-nLDL. Anticardiolipin antibodies (ACL) were also detected via ELISA. Compared to diabetic Coushatta men (n=14), diabetic Coushatta women (n=16) had a significantly higher median anti-oxLDL/anti-nLDL ratio (1.84 for women vs. 1.20 for men, p=0.017). A trend (p=0.050) towards higher median ratios were seen in non-diabetic women (n=21) relative to non-diabetic men (n=12). No significant differences were seen between diabetic and nondiabetic men, or between diabetic and nondiabetic women. In addition, no differences were seen in ACL, age, body mass index, or hemoglobin A1c between diabetic men and women. The data indicate that diabetic Coushatta women have higher levels of anti-oxLDL, and suggests that gender differences may act synergistically with the metabolic disease to increase levels of these antibodies.
|Original language||English (US)|
|Journal||Journal of Investigative Medicine|
|State||Published - Jan 1 1996|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)