Anti-MuSK autoantibodies block binding of collagen Q to MuSK

Y. Kawakami, M. Ito, M. Hirayama, K. Sahashi, B. Ohkawara, A. Masuda, H. Nishida, N. Mabuchi, Andrew G Engel, K. Ohno

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Abstract

Objective: Muscle-specific receptor tyrosine kinase (MuSK) antibody-positive myasthenia gravis (MG) accounts for 5%-15% of autoimmune MG. MuSK mediates the agrin-signaling pathway and also anchors the collagenic tail subunit (ColQ) of acetylcholinesterase (AChE). The exact molecular target of MuSK-immunoglobulin G (IgG), however, remains elusive. As acetylcholine receptor (AChR) deficiency is typically mild and as cholinesterase inhibitors are generally ineffective, we asked if MuSK-IgG interferes with binding of ColQ to MuSK. Methods: We used 3 assays: in vitro overlay of the human ColQ-tailed AChE to muscle sections of Colq-/- mice; in vitro plate-binding assay to quantitate binding of MuSK to ColQ and to LRP4; and passive transfer of MuSK-IgG to mice. Results: The in vitro overlay assay revealed that MuSK-IgG blocks binding of ColQ to the neuromuscular junction. The in vitro plate-binding assay showed that MuSK-IgG exerts a dosedependent block of MuSK binding to ColQ by but not to LRP4. Passive transfer of MuSK-IgG to mice reduced the size and density of ColQ tõ10% of controls and had a lesser effect on the size and density of AChR and MuSK. Conclusions: As lack of ColQ compromises agrin-mediated AChR clustering in Colq-/- mice, a similar mechanism may lead to AChR deficiency in MuSK-MG patients. Our experiments also predict partial AChE deficiency in MuSK-MG patients, but AChE is not reduced in biopsied NMJs. In humans, binding of ColQ to MuSK may be dispensable for clustering ColQ, but is required for facilitating AChR clustering. Further studies will be required to elucidate the basis of this paradox.

Original languageEnglish (US)
Pages (from-to)1819-1826
Number of pages8
JournalNeurology
Volume77
Issue number20
DOIs
StatePublished - Nov 15 2011

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Receptor Protein-Tyrosine Kinases
Autoantibodies
Collagen
Muscles
Cholinergic Receptors
Immunoglobulin G
Myasthenia Gravis
Acetylcholinesterase
Agrin
Cluster Analysis
Mouse
Neuromuscular Junction
Cholinesterase Inhibitors
Tail

ASJC Scopus subject areas

  • Clinical Neurology
  • Arts and Humanities (miscellaneous)

Cite this

Kawakami, Y., Ito, M., Hirayama, M., Sahashi, K., Ohkawara, B., Masuda, A., ... Ohno, K. (2011). Anti-MuSK autoantibodies block binding of collagen Q to MuSK. Neurology, 77(20), 1819-1826. https://doi.org/10.1212/WNL.0b013e318237f660

Anti-MuSK autoantibodies block binding of collagen Q to MuSK. / Kawakami, Y.; Ito, M.; Hirayama, M.; Sahashi, K.; Ohkawara, B.; Masuda, A.; Nishida, H.; Mabuchi, N.; Engel, Andrew G; Ohno, K.

In: Neurology, Vol. 77, No. 20, 15.11.2011, p. 1819-1826.

Research output: Contribution to journalArticle

Kawakami, Y, Ito, M, Hirayama, M, Sahashi, K, Ohkawara, B, Masuda, A, Nishida, H, Mabuchi, N, Engel, AG & Ohno, K 2011, 'Anti-MuSK autoantibodies block binding of collagen Q to MuSK', Neurology, vol. 77, no. 20, pp. 1819-1826. https://doi.org/10.1212/WNL.0b013e318237f660
Kawakami Y, Ito M, Hirayama M, Sahashi K, Ohkawara B, Masuda A et al. Anti-MuSK autoantibodies block binding of collagen Q to MuSK. Neurology. 2011 Nov 15;77(20):1819-1826. https://doi.org/10.1212/WNL.0b013e318237f660
Kawakami, Y. ; Ito, M. ; Hirayama, M. ; Sahashi, K. ; Ohkawara, B. ; Masuda, A. ; Nishida, H. ; Mabuchi, N. ; Engel, Andrew G ; Ohno, K. / Anti-MuSK autoantibodies block binding of collagen Q to MuSK. In: Neurology. 2011 ; Vol. 77, No. 20. pp. 1819-1826.
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AU - Ito, M.

AU - Hirayama, M.

AU - Sahashi, K.

AU - Ohkawara, B.

AU - Masuda, A.

AU - Nishida, H.

AU - Mabuchi, N.

AU - Engel, Andrew G

AU - Ohno, K.

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AB - Objective: Muscle-specific receptor tyrosine kinase (MuSK) antibody-positive myasthenia gravis (MG) accounts for 5%-15% of autoimmune MG. MuSK mediates the agrin-signaling pathway and also anchors the collagenic tail subunit (ColQ) of acetylcholinesterase (AChE). The exact molecular target of MuSK-immunoglobulin G (IgG), however, remains elusive. As acetylcholine receptor (AChR) deficiency is typically mild and as cholinesterase inhibitors are generally ineffective, we asked if MuSK-IgG interferes with binding of ColQ to MuSK. Methods: We used 3 assays: in vitro overlay of the human ColQ-tailed AChE to muscle sections of Colq-/- mice; in vitro plate-binding assay to quantitate binding of MuSK to ColQ and to LRP4; and passive transfer of MuSK-IgG to mice. Results: The in vitro overlay assay revealed that MuSK-IgG blocks binding of ColQ to the neuromuscular junction. The in vitro plate-binding assay showed that MuSK-IgG exerts a dosedependent block of MuSK binding to ColQ by but not to LRP4. Passive transfer of MuSK-IgG to mice reduced the size and density of ColQ tõ10% of controls and had a lesser effect on the size and density of AChR and MuSK. Conclusions: As lack of ColQ compromises agrin-mediated AChR clustering in Colq-/- mice, a similar mechanism may lead to AChR deficiency in MuSK-MG patients. Our experiments also predict partial AChE deficiency in MuSK-MG patients, but AChE is not reduced in biopsied NMJs. In humans, binding of ColQ to MuSK may be dispensable for clustering ColQ, but is required for facilitating AChR clustering. Further studies will be required to elucidate the basis of this paradox.

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