Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151

Ji Hye Kim, Mohamed Badawi, Jong Kook Park, Jinmai Jiang, Xiaokui Mo, Lewis Rowland Roberts, Thomas D. Schmittgen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells. miR-199a-3p reduced in vitro invasion and migration of CD151-positive HCC cells. Examination of the mRNA and protein expression in pairs of primary HCC tumors and adjacent benign tissues showed that not only was CD151 mRNA and protein increased in the tumors but also that an inverse correlation exists between the miR-199a-3p and CD151 RNA expression. We report that CD151 is a target of miR-199a-3p and that increased CD151 protein resulting from reduced miR-199a-3p could contribute to the development of metastatic HCC.

Original languageEnglish (US)
Pages (from-to)2037-2045
Number of pages9
JournalInternational Journal of Oncology
Volume49
Issue number5
DOIs
StatePublished - Nov 1 2016

Fingerprint

Hepatocellular Carcinoma
Proteins
Messenger RNA
Luciferases
Small Interfering RNA
Genes
Neoplasms
Glycoproteins
Western Blotting
Cell Proliferation
RNA
Cell Line

Keywords

  • Liver
  • Metastasis
  • MicroRNA
  • MiR-199a
  • Tspan24

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. / Kim, Ji Hye; Badawi, Mohamed; Park, Jong Kook; Jiang, Jinmai; Mo, Xiaokui; Roberts, Lewis Rowland; Schmittgen, Thomas D.

In: International Journal of Oncology, Vol. 49, No. 5, 01.11.2016, p. 2037-2045.

Research output: Contribution to journalArticle

Kim, Ji Hye ; Badawi, Mohamed ; Park, Jong Kook ; Jiang, Jinmai ; Mo, Xiaokui ; Roberts, Lewis Rowland ; Schmittgen, Thomas D. / Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. In: International Journal of Oncology. 2016 ; Vol. 49, No. 5. pp. 2037-2045.
@article{8203e0919fca4050adfee46bb98a9249,
title = "Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151",
abstract = "Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells. miR-199a-3p reduced in vitro invasion and migration of CD151-positive HCC cells. Examination of the mRNA and protein expression in pairs of primary HCC tumors and adjacent benign tissues showed that not only was CD151 mRNA and protein increased in the tumors but also that an inverse correlation exists between the miR-199a-3p and CD151 RNA expression. We report that CD151 is a target of miR-199a-3p and that increased CD151 protein resulting from reduced miR-199a-3p could contribute to the development of metastatic HCC.",
keywords = "Liver, Metastasis, MicroRNA, MiR-199a, Tspan24",
author = "Kim, {Ji Hye} and Mohamed Badawi and Park, {Jong Kook} and Jinmai Jiang and Xiaokui Mo and Roberts, {Lewis Rowland} and Schmittgen, {Thomas D.}",
year = "2016",
month = "11",
day = "1",
doi = "10.3892/ijo.2016.3677",
language = "English (US)",
volume = "49",
pages = "2037--2045",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151

AU - Kim, Ji Hye

AU - Badawi, Mohamed

AU - Park, Jong Kook

AU - Jiang, Jinmai

AU - Mo, Xiaokui

AU - Roberts, Lewis Rowland

AU - Schmittgen, Thomas D.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells. miR-199a-3p reduced in vitro invasion and migration of CD151-positive HCC cells. Examination of the mRNA and protein expression in pairs of primary HCC tumors and adjacent benign tissues showed that not only was CD151 mRNA and protein increased in the tumors but also that an inverse correlation exists between the miR-199a-3p and CD151 RNA expression. We report that CD151 is a target of miR-199a-3p and that increased CD151 protein resulting from reduced miR-199a-3p could contribute to the development of metastatic HCC.

AB - Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells. miR-199a-3p reduced in vitro invasion and migration of CD151-positive HCC cells. Examination of the mRNA and protein expression in pairs of primary HCC tumors and adjacent benign tissues showed that not only was CD151 mRNA and protein increased in the tumors but also that an inverse correlation exists between the miR-199a-3p and CD151 RNA expression. We report that CD151 is a target of miR-199a-3p and that increased CD151 protein resulting from reduced miR-199a-3p could contribute to the development of metastatic HCC.

KW - Liver

KW - Metastasis

KW - MicroRNA

KW - MiR-199a

KW - Tspan24

UR - http://www.scopus.com/inward/record.url?scp=84992677803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992677803&partnerID=8YFLogxK

U2 - 10.3892/ijo.2016.3677

DO - 10.3892/ijo.2016.3677

M3 - Article

C2 - 27599545

AN - SCOPUS:84992677803

VL - 49

SP - 2037

EP - 2045

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 5

ER -