Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1

I. Cohen, P. Boya, L. Zhao, D. Métivier, K. Andreau, J. L. Perfettini, J. G. Weaver, Andrew David Badley, E. W. Taylor, G. Kroemer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The third reading frame of the envelope gene from HIV-1 codes for a protein homologous to the human selenoprotein glutathione peroxidase (GPX). Cells stably or transiently transfected with a HIV-1 GPX construct are protected against the loss of the mitochondrial transmembrane potential and subsequent cell death induced by exogenous reactive oxygen species (ROS) as well as mitochondrion-generated ROS. However, HIV-1 GPX does not confer a general apoptosis resistance, because HIV-1 GPX-transfected cells were not protected against cell death induced by staurosporine or oligomycin. The inhibition of cell death induced by the ROS donor tert-butylhydroperoxide was also observed in cells depleted from endogenous glutathione (GSH), suggesting that GSH is not the sole electron acceptor for HIV-1 GPX. Clinical HIV-1 isolates from long-term non-progressors (untreated patients with diagnosed HIV-1 infection for >10 years, with CD4 T cell count of >500 cells/mm3) mostly possess an intact GPX gene (with only 18% of loss-of-function mutations), while HIV-1 isolates from patients developing AIDS contain non-functional GPX mutants in 9 out of 17 cases (53%). Altogether, these data suggest that HIV-1 GPX possesses a cytoprotective, pathophysiologically relevant function.

Original languageEnglish (US)
Pages (from-to)181-192
Number of pages12
JournalApoptosis
Volume9
Issue number2
DOIs
StatePublished - Mar 2004

Fingerprint

Glutathione Peroxidase
HIV-1
Cell death
Reactive Oxygen Species
Genes
Selenoproteins
Cell Death
tert-Butylhydroperoxide
Oligomycins
Mitochondria
Staurosporine
T-cells
Glutathione
glutathione peroxidase GPX1
Reading Frames
Apoptosis
Electrons
CD4 Lymphocyte Count
Membrane Potentials
HIV Infections

Keywords

  • Glutathione
  • Mitochondria
  • Selenium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Cohen, I., Boya, P., Zhao, L., Métivier, D., Andreau, K., Perfettini, J. L., ... Kroemer, G. (2004). Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1. Apoptosis, 9(2), 181-192. https://doi.org/10.1023/B:APPT.0000018800.87358.ba

Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1. / Cohen, I.; Boya, P.; Zhao, L.; Métivier, D.; Andreau, K.; Perfettini, J. L.; Weaver, J. G.; Badley, Andrew David; Taylor, E. W.; Kroemer, G.

In: Apoptosis, Vol. 9, No. 2, 03.2004, p. 181-192.

Research output: Contribution to journalArticle

Cohen, I, Boya, P, Zhao, L, Métivier, D, Andreau, K, Perfettini, JL, Weaver, JG, Badley, AD, Taylor, EW & Kroemer, G 2004, 'Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1', Apoptosis, vol. 9, no. 2, pp. 181-192. https://doi.org/10.1023/B:APPT.0000018800.87358.ba
Cohen I, Boya P, Zhao L, Métivier D, Andreau K, Perfettini JL et al. Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1. Apoptosis. 2004 Mar;9(2):181-192. https://doi.org/10.1023/B:APPT.0000018800.87358.ba
Cohen, I. ; Boya, P. ; Zhao, L. ; Métivier, D. ; Andreau, K. ; Perfettini, J. L. ; Weaver, J. G. ; Badley, Andrew David ; Taylor, E. W. ; Kroemer, G. / Anti-apoptotic activity of the glutathione peroxidase homologue encoded by HIV-1. In: Apoptosis. 2004 ; Vol. 9, No. 2. pp. 181-192.
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