Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells

Jorge A. Flores, Johannes D Veldhuis, Denis A. Leong

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca2+]i in a subpopulation of single swine granulosa cells. The Ca2+ source for this angiotensin II-mediated [Ca2+]i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca2+ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since folliclestimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca2+.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume81
Issue number1-3
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Granulosa Cells
Angiotensin II
Calcium
Swine
Video Microscopy
Guanine Nucleotides
Fura-2
Pertussis Toxin
Fluorescent Dyes
Carrier Proteins
Hormones
Ions

Keywords

  • Angiotensin II
  • Calcium release
  • Granulosa cell

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells. / Flores, Jorge A.; Veldhuis, Johannes D; Leong, Denis A.

In: Molecular and Cellular Endocrinology, Vol. 81, No. 1-3, 1991, p. 1-10.

Research output: Contribution to journalArticle

Flores, Jorge A. ; Veldhuis, Johannes D ; Leong, Denis A. / Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells. In: Molecular and Cellular Endocrinology. 1991 ; Vol. 81, No. 1-3. pp. 1-10.
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