Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells

Jorge A. Flores; Johannes D. Veldhuis; Denis A. Leong

doi:10.1016/0303-7207(91)90199-3

Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells

Jorge A. Flores, Johannes D. Veldhuis, Denis A. Leong

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The effects of angiotensin II on cytosolic free Ca²⁺ ion concentrations ([Ca²⁺]_i were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca²⁺]_i in a subpopulation of single swine granulosa cells. The Ca²⁺ source for this angiotensin II-mediated [Ca²⁺]_i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca²⁺ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since folliclestimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca²⁺.

Original language	English (US)
Pages (from-to)	1-10
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	81
Issue number	1-3
DOIs	https://doi.org/10.1016/0303-7207(91)90199-3
State	Published - Oct 1991

Keywords

Angiotensin II
Calcium release
Granulosa cell

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(91)90199-3

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title = "Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells",

abstract = "The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca2+]i in a subpopulation of single swine granulosa cells. The Ca2+ source for this angiotensin II-mediated [Ca2+]i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca2+ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since folliclestimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca2+.",

keywords = "Angiotensin II, Calcium release, Granulosa cell",

author = "Flores, {Jorge A.} and Veldhuis, {Johannes D.} and Leong, {Denis A.}",

note = "Funding Information: We thank Charles E. Lyons and Diana Juchter-Berry for their helpful technical advice, Dr. Erik Hewlett of the Departmento f Pharmacology for his generousg ift of purified pertussis toxin, the Smithfield abattoir for its cooperation and provision of ovarian tissue,M r. Amir Azimi for his dedicationa s carrier, and Patsy Craig for her skillful preparationo f the manuscript. This work was supportedi n part by NIH ROl HD16806a nd RCDA 1 K04 HD 00634( J.D.V.), the NSF Science Center for Biological Timing (J.A.F., J.D.V., D.A.L.) and the Hormone Action Training grant 5T32-DK07320( J.A.F.), and NIH R01 DK35937( D.A.L.).",

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N2 - The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca2+]i in a subpopulation of single swine granulosa cells. The Ca2+ source for this angiotensin II-mediated [Ca2+]i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca2+ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since folliclestimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca2+.

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Angiotensin II induces calcium release in a subpopulation of single ovarian (granulosa) cells

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